反相离子对色谱法测定青风藤中青藤碱的含量

目的建立离子对色谱法测定青风藤中青藤碱的含量。方法使用Eclipse Plus C18柱(4.6 mm×250 mm,5μm);乙腈:磷酸盐缓冲液(0.03 mol/L磷酸二氢钾和0.03 mol/L庚烷磺酸钠,磷酸调节pH值至4.0)=18:82为流动相,流速1.0 ml/min,柱温40℃,检测波长262 nm。结果青藤碱在0.025~0.5 mg/ml(r=1.000)范围内呈良好的线性关系。最低检测限为2.5μg/ml,低、中、高3个浓度的日内精密度RSD分别为0.62%、0.35%、0.60%,日间精密度RSD为1.54%、0.70%、0.45%。平均回收率99.9%(RSD=2.9%,n=6),均符合分析要求。结论该方法简单快捷,结果准确,适用于测定青风藤中青藤碱的含量。

Reversed-phase ion-pair chromatography in determination of sinomenine contents in Sinomenium acutum

Objective To establish a reversed-phase ion-pair chromatography (RP-IPC) method for determination of sinomenine content in Sinomenium acutum. Methods The IPC method was established using a column of Eclipse Plus C18 (4. 6 mm×250 mm, 5 μm) with a mobile phase of acetonitrile and buffer phosphate (0.03 mol/L potassium dihydrogen phosphate and 0. 03 mol/L sodium heptane sulfonic acid,adjusted to pH 4.0 by phosphoric acid)18 : 82. The flow rate was 1.0 ml/min;the detection wavelength was set at 262 nm, and the temperature was at 40℃. Results The linearity was obtained over a concentration range of 0.025-0.5 mg/ml(r= 1.000). The low limit of detection was 2.5μg/ml. The intra-day precisions of low, medium, and high concentrations of sinomenine were 1.54%, 0.70%, and 0.45%, and the inter-day precisions were 1.54% ,0.70% , and 0.45 % , respectively. The average recovery was 99.9% c RSD= 2.9 % , n= 6) , which met the requirement for analysis. Conclusion The present method is simple, fast, and accurate;it is suitable for determination of sinomenine content in Sinomenium acutum.