湿法超微粉碎-超滤法快速提取浓缩全蝎蛋白可行性考察

目的:考察湿法超微粉碎-超滤法快速提取浓缩全蝎蛋白的可行性。方法:利用大鼠体内抗凝血、抗血栓药效试验比较湿法超微粉碎-超滤法、胃蛋白酶酶解法2种提取工艺所得全蝎蛋白的药效差异,以考察湿法超微粉碎-超滤法用于全蝎蛋白提取浓缩的可行性。结果:2种提取方法所得全蝎蛋白均能明显延长大鼠凝血时间、血浆活化部分凝血酶时间、凝血酶原时间、凝血酶时间,并对大鼠动静脉血栓干、湿重均有明显减轻作用,与生理盐水对照组比较,均有统计学差异。结论:经大鼠体内抗凝血、抗血栓药效试验验证,采用湿法超微粉碎-超滤法快速提取浓缩全蝎蛋白是可行的。

Feasibility Investigation of Extraction and Concentration Technology for Protein from Buthus martensii Rapidly by Wet Superfine Grinding-Ultrafiltration Method

Objective:To investigate feasibility of extraction and concentration technology for protein from Buthus martensii rapidly by wet superfine grinding-ultrafiltration method. Method: Pharmacodynamic difference of protein from B. martensii was compared by in vivo anticoagulant and in vivo antithrombotic experiments in rats,which were prepared by two different extraction technology(wet superfine grinding-ultrafiltration method and pepsin enzymatic hydrolysis method),in order to investigate feasibility of extraction and concentration for protein from B. martensii by wet superfine grinding-ultrafiltration method. Result: Protein from B. martensii through two kinds of extraction methods could both significantly prolong blood coagulation time and plasma APTT,PT,TT of rats.Furthermore, they could reduce dry and wet weight of rat arteriovenous thrombosis significantly,compared with physiological saline control group,there were statistically significant difference. Conclusion: After approved by in vivo anticoagulant and in vivo antithrombotic experiments in rats,it was feasible of wet superfine grinding-ultrafiltration method used to extract and concentrate protein from B. martensii rapidly.