间质纤维母细胞的增殖活性在肺癌转移中的意义

目的：探讨纤维母细胞增殖活性与肺癌转移的关系。方法：采用免疫组织化学方法检测94例肺癌组织中纤维母细胞的增殖活性。结果：（1）肿瘤中心区纤维母细胞增殖细胞核抗原（PCNA）LI高于周围区纤维母细胞（P＜0．05）；癌旁肺组织中纤维母细胞PCNA LI明显低于肿瘤中心区和周围区纤维母细胞（P＜0．05）。（2）有转移组肿瘤中心区纤维母细胞和周围区纤维母细胞PCNA LI高于无转移组（P＜0．05）。结论：肿瘤中心区纤维母细胞的增殖活性是判断肺癌淋巴结转移或远处转移的有价值的参考指标。

Implications of Proliferative Activity of Intratumoral Fibroblasts in the Metastasis of Lung Carcinomas

ve To evaluate the correlation between the proliferative activity of fibroblasts and the metastasis of lung carcinomas. Methods 94 cases of surgically resected specimens of lung carcinomas were collected. The proliferative activity of fibroblasts was evaluated with the mouse monoclonal antibody against pro liferating cell nuclear antigen by immunohistochemical method. Results The PCNA LI of tumor cells in the outer area was higher than that in the inner area, and the PCNA LI of fibroblasts in the inner area was signifi cantly higher than that of fibroblasts in the outer area . The PCNA LI of fibroblasts in the nontumor area was sig nificantly lower than that of fibroblasts in the inner or outer areas. There was a close association between the PC NA LI of fibroblasts and the PCNA LI of tumor cells in the inner or outer area. The high PCNA LI of fibroblasts in the inner area showed a significant correlation with lymph node metastasis of lung carcinomas. Conclusions This study demonstrated that there is a discrepancy in the distribution of highly proliferative tumor cells and highly proliferative fibroblasts in lung carcinomas. The proliferative activity of tumor cells should be assessed in tumor cells in the outer area, and that of fibroblasts should be assessed in fibroblasts in the inner area. The pres ent study also indicated that the metastatic ability of lung carcinomas is closely dependent on proliferative activi ty of fibroblasts in the inner area.