XIAP and P-glycoprotein co-expression is related to imatinib resistance in chronic myeloid leukemia cells |
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| Authors: | Karina Lani Silva Paloma Silva de Souza Gabriela Nestal de Moraes Arthur Moellmann-Coelho Flavia da Cunha Vasconcelos Raquel Ciuvalschi Maia |
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| Institution: | 1. Laboratório de Hemato-Oncologia Celular e Molecular, Programa de Hemato-Oncologia Molecular, Coordenação Geral Técnico-Científica (CGCT), Instituto Nacional de Câncer (INCA) and Programa de Pós-Graduação em Oncologia/INCA, Brazil;2. Serviço de Hematologia, Hospital do Câncer I, INCA, Brazil |
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| Abstract: | P-glycoprotein (Pgp) and XIAP co-expression has been discussed in the process of the acquisition of multidrug resistance (MDR) in cancer. Here, we evaluated XIAP and Pgp expression in chronic myeloid leukemia (CML) samples, showing a positive correlation between them. Furthermore, we evaluated the effects of imatinib in XIAP and Pgp expression using CML cell lines K562 (Pgp−) and K562-Lucena (Pgp+). Imatinib increased XIAP and Pgp expression in K562-Lucena cells, while in K562 cells a downregulation of these proteins was observed, suggesting that imatinib induces an increment of MDR phenotype of CML cells that previously exhibit high levels of Pgp/XIAP co-expression. |
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| Keywords: | CML chronic myeloid leukemia Pgp P-glycoprotein MDR multidrug resistance IAPs inhibitor of apoptosis proteins RFI ratio of mean of fluorescence intensity MFI mean of fluorescence intensity Rho-123 rhodamine-123 CSA cyclosporin A VRP verapamil IC50 concentration achieving 50% inhibition of cell growth |
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