Fas/APO-1基因转染食管癌Eca-109细胞及其表达

 目的　通过将Fas/APO 1基因转染食管癌细胞 ,比较转导前后基因及蛋白的表达水平。方法　运用已构建的Fas/APO 1真核表达质粒 ,用脂质体介导法将目的基因导入Eca 10 9细胞 ,筛选克隆细胞以Southernblot ,Northernblot ,Westernblot法检测Fas/APO 1基因的表达。结果　转导株与非转导株均有Fas/APO 1cDNA的表达 ,但转导株在基因及蛋白水平的表达均明显高于非转导株。结论　Fas/APO 1基因在食管癌细胞中处于低表达状态 ;通过基因转导能有效增强其表达。

Expression of Fas/APO -1 in human esophageal cancerous cells transducted with Fas/APO -1 gene

Objective To compare the expressing level of Fas/APO \|1 gene in gene transfected and not transfected esophageal cancer cells through transducting Fas/APO \|1 gene into esophageal cancer cells. Methods The reconstructed plasmid expressing Fas/APO \|1 gene with lipofectamine was transducted into esophageal cancer cell line Eca\|109. Then the positive clones were chosen and the expressing level of Fas/APO \|1 gene was determined by the means of Southern blot, Northern blot and Western blot. Results The blotting results showed that Fas/APO \|1 cDNA was expressed in both gene transducted and not transducted cell strains, but the expressing level of Fas/APO \|1 mRNA and protein in gene transducted cell strain was much higher than that in gene not transducted cell strain. Conclusion The expressing level of Fas/APO \|1 gene, which was very low in esophageal cancer cells, could be enhanced by the means of gene transduction efficiently.