Vasopressin-stimulated Ca2+ reabsorption in rabbit cortical collecting system: effects on cAMP and cytosolic Ca2+ |
| |
Authors: | J van Baal Gertraud Raber J de Slegte Reagan Pieters René J M Bindels P H G M Willems |
| |
Institution: | (1) Department of Cell Physiology, University of Nijmegen, PO Box 9101, NL-6500 HB Nijmegen, The Netherlands, NL;(2) Department of Biochemistry, University of Nijmegen, PO Box 9101, NL-6500 HB Nijmegen, The Netherlands, NL |
| |
Abstract: | The effect of arginine vasopressin (AVP) on transepithelial Ca2+ transport in primary cultures of rabbit cortical collecting system cells was examined. Addition of AVP to the basolateral
side of the monolayer dose-dependently (EC50 = 0.7 nM) increased active Ca2+ reabsorption from a basal value of 85 ± 2 nmol·h–1·cm–2 to a maximum value of 124 ± 3 nmol·h–1·cm–2. This was paralleled by a dose-dependent (EC50 = 1.1 nM) increase in cellular adenosine 3′,5′-cyclic monophosphate (cAMP) content. Both effects of AVP were mimicked by
the V2 agonist deamino-Cys,D-Arg8-vasopressin (dDAVP) and forskolin. Addition of either AVP or dDAVP to the basolateral side evoked a sustained increase in
cytosolic free Ca2+ concentration, which resulted from both Ca2+ entry and release from internal stores. Only the effect on Ca2+ entry was mimicked by forskolin, demonstrating that cAMP acts by activating a Ca2+ influx pathway. The present findings demonstrate that AVP stimulates transcellular Ca2+ transport in the cortical collecting system through activation of basolateral V2 receptors coupled to adenylyl cyclase to increase the cellular cAMP content.
Received: 4 July 1996 / Received after revision and accepted: 3 September 1996 |
| |
Keywords: | Connecting tubule Cortical collecting duct Arginine vasopressin Ca2+ transport |
本文献已被 SpringerLink 等数据库收录! |
|