| 高效液相色谱法测定跌打止痛巴布膏中大黄酚的含量 |
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| 引用本文： | 汪小根,黄艳萍,邹玉繁.高效液相色谱法测定跌打止痛巴布膏中大黄酚的含量[J].中国药房,2005,16(17):1332-1333. |
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| 作者姓名： | 汪小根 黄艳萍 邹玉繁 |
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| 作者单位： | 广东化工制药职业技术学院,广州市,510520 |
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| 摘 要： | 目的:建立以高效液相色谱法测定跌打止痛巴布膏中大黄酚含量的方法。方法:色谱柱为KromasasilC18,流动相为甲醇-0.1%磷酸水(85∶15),流速为1.0ml/min,检测波长为254nm,进样量为10μl。结果:大黄酚进样量在0.6μg～4μg范围内与峰面积值线性关系良好(r=0.9994);平均加样回收率为99.23%(n=5,RSD=2.45%)。结论:本方法准确、可靠、重现性好。
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| 关 键 词： | 高效液相色谱法 跌打止痛巴布膏 大黄酚 含量 |
| 文章编号： | 1001-0408（2005）17-1332-02 |
| 收稿时间： | 2004-09-15 |
| 修稿时间： | 2005-01-15 |
Determination of Chrysophanol in Diedazhitong Cataplasm by HPLC |
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| WANG Xiaogen,Huang Yanping,ZOU Yufan.Determination of Chrysophanol in Diedazhitong Cataplasm by HPLC[J].China Pharmacy,2005,16(17):1332-1333. |
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| Authors: | WANG Xiaogen Huang Yanping ZOU Yufan |
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| Abstract: | OBJECTIVE:To determine the content of chrysophanol in Diedazhitong cataplsam by HPLC.METHODS:The samples were separated on a Kromasasil C 18 ,methol-0.1%phosphoric acid(85∶15)was taken as the mobile phase with a flow rate at1.0ml/min and detection wavelength at254nm,the sample size was10μl.RESULTS:Good linear relationship was achieved when the concentration range of chrysophanol was0.6μg～4μg(r=0.9994);The average recovery of chrysophanol was99.23%(n=5,RSD=2.45%).CONCLUSION:This method is accurate,reliable,and reproducible. |
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| Keywords: | HPLC Diedazhitong cataplasm Chrysophanol Content |
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