高效液相色谱法测定肠内制剂中的谷氨酰胺含量

目的建立一种准确、简便、快速的高效液相色谱法用于测定肠内制剂中谷氨酰胺的含量。方法以邻苯二甲醛作柱前衍生剂，反相C18柱为固定相，以10mmol／L磷酸盐缓冲液（pH6．85）：甲醇=50：50为流动相进行洗脱，在340nm紫外波长处测定谷氨酰胺的含量。结果在100～1500μmol／L内线性关系良好（r=0．9995）。回收率为87．37％～100．99％。结论该方法简便、准确，适用于肠内制剂中谷氨酰胺含量的分析。

Determination of Glutamine in Enteral Nutrient by High Performance Liquid Chromatography

Objective To establish a new high performance liquid chromatography （HPLC） method for determining the contents of glutamine （Gin） in enteral nutrient. Methods Gin was derived with O-phthalaldehyde and eluted with 10 mmol/L phosphate buffer （ pH 6.85） containing methanol on reverse phase C18 column. The derivative was detected at 340 nm ultraviolet light. Results The calibration curves of Gin was linear from 100 to 1 500 μmoL/L （r =0. 9995） , and the recovery ratio was 87.37%-100.99%. Conclusions This method is simple, rapid, and effective. It can be used to determine Gin contents of enteral nutrient.