| 化学发光免疫分析法与酶联免疫吸附法乙肝表面抗原的结果对比 |
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| 引用本文： | 黄晓辉,黄志昂,高贵阳,林晓璇.化学发光免疫分析法与酶联免疫吸附法乙肝表面抗原的结果对比[J].中医临床研究,2020(14):114-116. |
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| 作者姓名： | 黄晓辉 黄志昂 高贵阳 林晓璇 |
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| 作者单位： | 揭阳市榕城区中心医院 |
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| 摘 要： | 目的:比较化学发光免疫分析法(Chemiluminescence Immunoassay,ECLIA)与酶联免疫吸附法(Enzyme-Linked Immuno Sorbent Assay,ELISA)检测乙肝表面抗原(Hepatitis B Surface Antigen,HBsAg)的灵敏度与特异性。方法:选取2018年9月-2019年8月本院传染科收治的150例疑似乙型肝炎患者,采集所有入选者血液样本,对其进行ECLIA法和ELISA法检测。结果:ECLIA法的阳性检出率为高达93.3%(140/150),ELISA法阳性率仅仅为74.7%(112/150),ECLIA法阳性率明显高于ELISA法,与ELISA法比较差异有统计学意义(P<0.05);故ECLIA法灵敏度明显高于ELISA法(P<0.05)。结论:与ELISA法相比,ECLIA法对HBsAg检测在阳性率,灵敏度等许多方面都有比较显著的优势,故建议将ECLIA法作为临床检测HBsAg的首先方法而推广应用。
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| 关 键 词： | 化学发光免疫分析法 酶联吸附免疫法 乙肝表面抗原 灵敏度 特异性 |
Comparison of results between ECLIA and ELISA for hepatitis B surface antigen |
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| Abstract: | Objective:To compare the sensitivity and specificity of ECLIA and ELISA in detecting HBsAg.Method:150 patients with suspected hepatitis B were detected by ECILA and ELISA respectively,those results were compared.Results:HBsAg positive rate of ECILA was 93.3%(140/150),significantly higher than 74.7%(112/150)of ELISA,with statistically significant difference(P<0.05).The sensitivity of ECILA was significantly higher than ELISA(P<0.05).Conclusion:Compared with ELISA method,ECILA method for detecting HBsAg in positive rate,sensitivity and specificity of existence has obvious advantages,therefore,ECILA method was proposed as the preferred method of detecting HBsAg. |
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| Keywords: | ECILA ELISA HBsAg Sensitivity Specificity |
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