从免疫炎性损伤角度探讨5种清热解毒药物抗流感的机制及其临床意义

目的探讨黄芩、板蓝根、白头翁、虎杖、白花蛇舌草5种清热解毒代表药物抗流感病毒感染所致免疫炎性损伤的作用机制。方法在流感病毒亚洲甲型鼠肺适应株FM1感染小鼠后的不同时相(初期、极期、后期),采用ELISA法,动态观察清热解毒代表药物对小鼠血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、γ干扰素(IFN-γ)5种炎性细胞因子含量的影响。结果黄芩在感染后第3日可显著降低血清TNF-α、IL-1、IL-6含量,并于感染后各时相均可增加IFN-γ含量,感染后第1~5日增加IL-10含量。板蓝根在感染后第3日能显著降低血清TNF-α含量,第3~5日降低IL-6含量,并在感染后第1天升高IL-10含量,在感染后第3~5日能升高IFN-γ含量。白头翁在感染后第3日能明显降低血清TNF-α、IL-1、IL-6含量,同时显著升高IL-10含量。虎杖在感染后第1日可显著升高血清IL-10含量,在感染后第5日升高IFN-γ含量。白花蛇舌草在感染后第3日降低血清IL-6含量,在感染后第5日升高IFN-γ含量。结论黄芩的抗流感作用主要表现在流感病毒感染引起的急性外感热病的极期;板蓝根在流感病毒感染的各个时相均有一定的治疗作用;白头翁在流感病毒感染的极期可抑制炎性损伤;虎杖在感染流感病毒的初期与后期均可减少促炎性细胞因子的分泌;白花蛇舌草在流感病毒感染的极期及后期均可起到一定的抗免疫炎性损伤。各药物均具有抑制炎性损伤、防止多脏器功能衰竭、防止休克、退热和抑制促炎性细胞因子的分泌,调节机体免疫,从而减轻炎症反应的作用。

Study on the Mechanism of Anti-influenza of Five Kinds of Heat-clearing and Detoxifying Drugs from the Perspective of Immunoinflammatory Injury and Its Clinical Significance

Objective:To investigate the mechanism of action of five kinds of clearing and detoxifying drugs on behalf of Scutellaria,Radix,Chinese bulbul,Polygonum cuspidatum,diffusa against influenza virus infection-induced immune inflammatory injury.Methods:The effects of the representative drugs of clearing away heat and detoxification were observed on the contents of TNF-α,IL-1,IL-6,IL-10 and IFN-γin different phases of FM1 infection in mice after the Asian influenza virus influenza rat lung adapted strains with ELISA.Results:Scutellaria on 3 rd day after infection significantly reduced serum TNF-α,IL-1 and IL-6 content,and increased IFN-γcontent at each time and after infection,IL-10 on 1 to 5 days after infection.Banlangen could significantly reduce TNF-αcontent on the 3 rd day,decrease IL-6 content on the 3 rd^5 th day,increase IL-10 content on the 1 st day and increase IFN-γcontent on the 3 rd-5 th day after infection.On the third day after infection,the content of TNF-α,IL-1 and IL-6 in serum were significantly decreased,while the content of IL-10 was significantly increased due to Chinese bulbul.On the first day after infection,the level of serum IL-10 and IFN-γincreased significantly due to Polygonum cuspidatum.The content of IL-6 in serum decreased on the 3 rd day and IFN-γincreased on the 5 th day after infection due to diffusa.Conclusion:The anti-influenza effect of Scutellaria baicalensis Georgi is mainly manifested in the extreme phase of acute exogenous fever caused by influenza virus infection.Isatis root has a certain effect on the treatment of influenza in all phases of influenza virus infection.In the polar stage of influenza virus infection,Pulsatilla can inhibit the inflammatory damage.Polygonum cuspidatum can reduce the secretion of pro-inflammatory cytokines in the early and late stage of influenza virus infection.Hedyotis diffusa can play a certain role in anti-inflammatory damage in the polar and later stage of influenza virus infection.All the drugs have the functions of inhibiting inflammatory injury,preventing multiple organ failure,preventing shock,antipyretic,inhibiting the secretion of pro-inflammatory cytokines,regulating the body′s immunity,so as to reduce the inflammatory response.