桃叶珊瑚苷舒张血管作用机制研究

目的探讨桃叶珊瑚苷舒张血管的作用机制。方法去甲肾上腺素(10-6 mol/L)收缩内皮完整、去内皮、加入一氧化氮合酶抑制剂L-NAME孵育的大鼠胸主动脉血管后,加入累积浓度的桃叶珊瑚苷,检测血管张力变化。桃叶珊瑚苷孵育大鼠心脏微血管内皮细胞24 h,应用MTT法检测细胞活力,应用ELISA法检测细胞eNOS蛋白表达。结果桃叶珊瑚苷(0.5,5,20μmol/L)具有舒张内皮完整的大鼠胸主动脉血管的作用,去内皮和L-NAME (100μmol/L)能够抑制桃叶珊瑚苷舒张血管的作用。桃叶珊瑚苷(0.5,5,20μmol/L,24 h)具有促进大鼠心脏微血管内皮细胞eNOS蛋白表达的作用。结论桃叶珊瑚苷可能通过内皮依赖的NO途径发挥快速的舒张血管的作用,并且具有促进eNOS蛋白表达的作用。

Study on the Mechanism of Vasodilatory of Aucubin

Objective To study the vasorelaxation effect of aucubin and its Mechanism.Methods Rat thoracic aortas relaxed by aucubin with various concentrations after contracted by NE(10-6mol/L).Vascular tension was detected by tension transducer.Rat cardiac microvascular endothelial cells were incubated with aucubin for 24 h.Cell viability was detected by MTT method.eNOS protein expression was detected by ELISA.Results Aucubin(0.5,5,20μmol/L)has the effect of diastolic endothelial intact rat thoracic aortic vessels,and deendothelialization and L-NAME(100μmol/L)are able to inhibit the effect of aucubinn diastolic vessels.aromoside(0.5,5,20μmol/L,24 h)promoted the expression of eNOS protein in rat heart microvascular endothelial cells.Conclusion Aucubin could lead to relaxation in rat aortic rings.NO pathway might be involved in the endothelium-dependent relaxatio.Chronic aucubin treatment could increase eNOS expression in endothelial cells.