左旋多巴和姜黄素共递送protocells纳米粒的制备及体外评价

目的 制备共载左旋多巴和姜黄素 protocells 纳米粒并进行体外评价.方法 以介孔二氧化硅为内核,脂质双分子层为外膜,制备共载左旋多巴和姜黄素 protocells 纳米粒.使用激光粒度分析仪和透射电子显微镜对所制备纳米粒的形貌、粒径、多分散系数(PDI)和 Zeta 电势进行表征;采用高效液相色谱法对所制备纳米...

Preparation and in vitro evaluation of levodopa and curcumin co-loaded protocells nanoparticles

Objective We aim to prepare and evaluate the levodopa and curcumin co-loaded protocells nanoparticles in vitro.Methods Levodopa and curcumin co-loaded protocells nanoparticles were prepared with mesoporous silica nanoparticles(MSNs)as core and lipid bilayer as outer membrane.The morphology,particle size,polydispersion index(PDI)and Zeta potential of the nanoparticles were characterized by laser particle size analyzer and transmission electron microscope(TEM).The drug loading capacity(DLC)and entrapment efficiency(EE)of the nanoparticles were determined by HPLC.The in vitro release characteristics of the nanoparticles were investigated by dialysis bag method.The storage stability of the nanoparticles at room temperature was evaluated by particle size,Zeta potential and so on.Results The particle size distribution of levodopa and curcumin co-loaded protocells nanoparticles was uniform with the average particle size of(210.9±2.8)nm and PDI of(0.201±0.011),and the surface of the particles was electronegative.The DLC and EE of levodopa were(20.28±0.43)%and(10.14±0.22)%,respectively.The DLC and EE of curcumin were(1.97±0.01)%and(98.32±0.01)%,respectively.The results of in vitro release showed that the cumulative release rate of curcumin was 59.2%in 48 h,and the exposure amount of levodopa was significantly reduced in the circulatory system.The results of stability showed that levodopa and curcumin were stable in the nanoparticles.Conclusion The preparation process of levodopa and curcumin co-loaded protocells nanoparticles is simple,which have good physicochemical properties,stability and expected release properties.