术前化疗后结直肠癌组织中Fas/FasL和survivin的表达

目的探讨化疗后结直肠癌组织Fas/FasL和survivin的表达情况及两者之间的相关性。方法采用脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记技术(TUNEL法)和免疫组化方法,测定化疗后和未化疗两组各16例结直肠癌患者肿瘤的原位凋亡(AI)、原位增殖(KI)及survivin表达,运用流式细胞仪测定肿瘤细胞的细胞周期、Fas/FasL表达,用双抗体ELISA法检测患者血清可溶性Fas/FasL(sFas/sFasL)表达。结果化疗组结直肠癌患者肿瘤细胞的AI及Fas/FasL均较对照组高,KI、G2/M期细胞、sFas及survivin较对照组低,差别有统计学意义(P<0.05)。结论化疗通过诱导结直肠癌细胞凋亡及阻止细胞进入G2/M期从而抑制癌细胞的生长。Fas/FasL系统与结直肠癌细胞凋亡、增殖调控有关。survivin阳性表达下降程度可作为化疗有效的标志之一。

Fas/FasL and survivin expression in colorectal cancer cells after preoperative chemotherapy

Objective:To investigate the expressions of Fas/FasL and survivin in colorectal cancer cells after chemotherapy and the correlationship between them. Methods:Thirty-two colorectral cancer patients were divided into two groups randomly. One group ( n =16) received chemotherapy and the control group( n =16) did not. The apoptotic cells index (AI) in situ was identified by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assay. Immunohistochemical method was used for measurement of Ki67 proliferation index (KI) and survivin expression. Cell cycle and Fas/FasL expression were assessed by Flow Cytometry. The serum soluble Fas/FasL (sFas/FasL) was determined by enzyme linked immunosorbent assay (ELISA). Results:The AI and Fas/FasL expression were higher in the chemotherapy group than in the control group. The KI,G_2/M cycle arrest, sFas and survivin expression were lower in the chemotherapy group than in the control group. The difference was significant( P <0.05). Conclusions:Preoperative chemotherapy can effectively inhibit colorectal cancer cells proliferation by inducing the apoptosis and G_2/M arrest of the cancer cells at G_2/M stage. Fas/FasL system is related with apoptosis and proliferation of colorectal cancer cells. Survivin expression provides prognostic information that may have important therapeutic implications.