神经干细胞移植对大鼠视神经部分损伤后视觉诱发电位的影响

目的观察研究神经干细胞(NSCs)移植入视神经部分损伤SD大鼠后闪光视觉诱发电位(F-VEP)的变化。方法24只健康成年SD大鼠随机分为NSCs移植组(N组)和对照组(C组),每组12只大鼠,两组均使用精确校准方法在大鼠右眼造成部分视神经损伤,左眼为正常对照。从胚胎SD大鼠海马分离NSCs,利用细胞培养和体内移植技术,将培养后的NSCs注入视神经损伤后N组大鼠玻璃体内,C组大鼠视神经损伤眼玻璃体内注入同等体积的PBS。以上两组分6个时间段,即损伤前、损伤时、损伤后1周、2周、3周、4周分别检测损伤视神经眼的F-VEP,记录P1波幅及峰潜时,并进行统计分析。结果N组及C组P1波幅随时间延长均不同程度降低,但前者趋势较后者缓和。自第2周开始N组波幅均高于C组,且差异有显著性；N组及C组P1峰潜时均随时间变化,在第3周时达到最长,第4周时P1峰潜时有缩短；自第1周开始N组峰潜时均较C组缩短,且差异均有显著性意义。结论NSCs移植入视神经部分损伤大鼠可部分改善视神经传导功能。

Effect on flash visual evoked potential by neural stem cells after transplanted into the rats with partial optic nerve injury

Objective To investigate the changes of flash visual evoked potential (F-VEP) of the Sprague-Dawly (SD) rats with partial optic nerve injury after transplanted with neural stem cells (NSCs). Methods Twenty-four healthy adult SD rats were randomly divided into 2 groups (n=12):NSCs transplantation group (N) and control group (C).Their right eyes as model were injured partially by calibrated optic nerve crush,and the left eyes served as controls.NSCs were taken from embryonic rat hippocampus and cultured in vitro and grafted in vivo into the injured eyes of group N.The injured eyes of group C were injected with PBS of the same volume as group N.And the F-VEP was recorded in all rats before crush,0,1,2,3 and 4 weeks after crush.The statistical analysis was done in P1 wave amplitude and wave latency.Results The P1 wave amplitude of both group N and C was decreased as the time went on, but the decreasing trend of group N was more gentle than that of group C;since week 2,the P1 wave amplitude of group N was distinctly higher than that of group C.The P1 wave latency was delayed before 3 weeks and shortened at week 4.Since week 1,the P1 wave latency of group N was evidently shorter than that of group C.Conclusion The transplantation of NSCs after optic nerve crush can partly improve the function of visual nerve conduction.