L-肉碱在奥硝唑所致大鼠睾丸和附睾损伤中的保护作用

目的:探讨L-肉碱(LC)在奥硝唑(ORN)所致大鼠附睾和睾丸损伤中的的保护作用。方法:40只雄性SD大鼠(200～230g)随机均分为5组:①A组:给予0.5%的羧甲基纤维素钠(溶剂)灌胃;②B组:每天给予400mg/kgORN灌胃;③C组:每天给予800mg/kgORN灌胃;④D组:每天给予ORN(400mg/kg)+LC(100mg/kg)]灌胃;⑤E组:每天给予ORN(800mg/kg)+LC(100mg/kg)]灌胃。上述各组均连续灌胃20d,末次给药24h后,所有大鼠麻醉后处死,分别取睾丸、附睾,进行称重和HE染色,计算睾丸、附睾系数并观察睾丸和附睾病理组织学改变。结果:①与A组相比,B组睾丸、附睾系数明显降低(P<0.05);而C组睾丸、附睾系数为极显著性降低(P<0.01);D组与A组相比无差异,E组与A组相比有极显著性差异(P<0.01);②HE染色显示,与A组相比,B组睾丸生精小管内各级生精细胞排列基本整齐,部分生精小管管腔内有脱落的生精细胞,附睾管腔中精子数目下降,有时可见散在的生精细胞;C组大鼠睾丸生精小管管腔内均可见坏死脱落的生精细胞,附睾管腔中精子数目明显减少,且有较多的非精子细胞成分。D组睾丸生精小管无明显改变,附睾管腔中精子数目也未见明显下降;E组睾丸生精小管管腔内精子数目减少,可见坏死脱落的生精细胞,附睾腔中精子数目明显减少,并伴有较多的非精子细胞成分。结论:奥硝唑(ORN)可导致雄性大鼠附睾和睾丸病理组织学改变,LC对ORN引起大鼠附睾和睾丸损伤具有一定的保护作用。

Protective Effect of L-Carnitine on the Testis and Epididymis Against Ornidazole-Induced Injury in Male Rats

Objective:To investigate the protective effect of L-carnitine on the testis and epididymis against ornidazole(ORN)-in-duced injury in male rats.Methods:Forty male SD rats weithing 200-230 g were randomly divided into 5 groups,Group A treated with 0.5% sodium carboxymethyl cellulose,and Groups B,C,D and E with ORN at the daily dose of 400 mg/kg,800 mg/kg,400mg/kg plus LC 100 mg/kg and 800 mg/kg plus LC 100 mg/kg,respectively,all by oral gavage for 20 days continuously.Twenty-four hours after the last administration,all the rats were put to death,their testes and epididymides harvested,weighed and subjected to HE staining.The indexes of the testes and epididymides were obtained and their histopathological changes observed. Results:Compared with Group A,Groups B and C showed significant decreases in the indexes of the testis and epididymis(P<0.05 and P<0.01),while Group D exhibited no difference and Group E extremely significant difference(P<0.01).HE staining revealed that the sper-matogenic cells at all levels of testicular seminiferous tubules were neatly arranged in Group B,caducous in some seminiferous tubules,with decreased number of sperm and sporadic spermatogenic cells in the epididymal duct.Necrotic and caducous spermatogenic cells were observed in the seminiferous tubules of Group C,with significantly decreased number of sperm and lots of non-sperm cell compo-nents in the epididymal duct.No obvious changes were found in the testicular seminiferous tubules,nor evident reduction in the num-ber of sperm in the epididymal duct of Group D.Group E showed decreased number of sperm in the testicular seminiferous tubules,necrotic and caducous spermatogenic cells.obviously reduced number of sperm and a lot of non-sperm cell components in the epididy-mal duct.Conclusion:ORC can induce histopathological changes in the testis and epididymis of male rats,and L-carnitine plays a role in protecting the testis and epididymis from ORN-induced injury in male rats.