TECAI型生物人工肝支持系统治疗急性肝衰竭犬的实验研究

目的评价经改进的TECAI型生物人工肝脏支持系统（bioartificial liver supportsyst em,BALSS）治疗醋氨酚诱发急性肝衰竭(acute liver failure,ALF)犬的有效性和安全性。方法采用多次皮下注射醋氨酚的方法建立ALF模型犬。分离中国实验用小型猪肝细胞并培养于BALSS中，对ALF犬进行6小时的治疗，观察治疗前后犬生理、生化和组织学的变化，与常规药物治疗组和对照组进行比较。结果注射醋氨酚48小时后，可建立ALF犬模型，模型成功率为63.16%。应用我们改进的酶消化法，平均从每只小型猪的肝脏可得到（0.8～3.0）×10

Experimental study of type TECAI bioartificial liver suppert system to treatment of acute liver failure in a canine model

Objective:To assess the efficacy and safety of reformed typeTECAI bioartificial liver support system(BALSS) on treatment of acute liver failure(ALF)in a canine model induced by acetaminophen. Methods:The ALF canine model by multiple subcutaneous injections of acetaminophen were replicated. The primary hepatocytes were separated from the swine and cultured within the BALSS. The ALF model was treated with BALSS for six hours, the changes in physiological,biochemical and histological parameters were observed before and after the treatment ,and compared them with that after drugs treatment and control groups. Results:The ALF canine model was developed 48 hours after administrate of acetaminophen, the successful rate of model was 63.16%. Using our modified enzymatic digestion method, the yield of hepatocytes was (0.83.0)×1010 from per swine with high viability. Type TECAI BALSS treatment resulted in beneficial effects with decrease in plasma NH3, alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) levels decreased,and the ratio of branchchain amino acid to aromatic amino acid(BCAA/AAA) increased. The pathological changes of the liver were repaired,and the animals with ALF could live longer than 30 days. The conditions of ALF models after the coventional treatment and control groups deteriorated,and they died within 14.8360.00 hours. Conclusions:This type of BALSS is rapid,safe and efficacious in providing liver support in the ALF canine model.The efficacy of BALSS is superior to the routine drugs treatment,and it would hopefully be a new method in the treatment of ALF.