肿瘤抑制基因 p16 在贲门癌变过程中的表达

目的探讨肿瘤抑制基因 p16 的表达在贲门癌发生发展中的作用.方法对来自贲门癌高发区的 135 例贲门各级病变组织采用免疫组织化学方法进行测定.结果贲门上皮各级病变组织均出现不同程度的 p16 蛋白免疫阳性反应.且随着贲门上皮病变的加重,p16 蛋白免疫阳性率呈下降趋势,异形增生上皮和腺癌与贲门炎症上皮比较差异有显著意义.高、中分化腺癌的表达率(50.0%)显著高于低分化腺癌(22.2%),P<0.05.结论p16 基因的失活与贲门癌发生有关,并与贲门癌的组织分化程度有关.

Expression of Tumor Suppressor Gene p16 in Gastric Cardia Carcinogenesis

Objective To explore the role of tumor suppressor gene p16 in the carcinogenesis and progression of gastric cardia carcinoma.Methods One hundred and thirty five cases of gastric cardia epithelium in the different stages lesions from high incidence area of gastric cardia cancer were examined with immunohistochemistry method.Results The different degrees of immunostaining of p16 occurred on gastric cardia epithelium.With the lesions progressing,the positive immunostaining rate of p16 protein had a decreasing tendency.The positive rate in dysplasia and adenocarcinoma was much lower than inflammation epithlium, P <0 05.The positive rate of p16 protein was 50% in the well differentiated adenocarcinomas and 22 2% in poorly differentiated ones.The difference was significant between the two groups.Conclusions Loss of p16 protein expression is closely associated with the initiation and differentiated pattern of gastric cardia carcinoma.