大鼠自体原位肾移植缺血再灌注损伤模型的建立

目的 :建立一种简便的大鼠自体原位肾移植模型 ,以便于研究肾移植后缺血再灌注损伤 (IRI)与肾移植后肾脏损伤的关系。方法 :雄性SD大鼠 2 0只 ,自体原位肾移植组 (A组 )和假手术组 (B组 )各 10只。左腰部纵切口 ,采用显微外科技术在后腹膜间隙游离左侧输尿管及肾脏、肾蒂。A组 :以无损伤动脉夹闭合肾动脉 ,采用管径0 .2mm的玻璃穿刺针经肾动脉恒压 ( 10 0mmHg)灌注 1ml 0～ 4℃生理盐水 ,至肾脏颜色变白后暂时阻断肾动、静脉 ,吻合肾动脉切口 ,采用自制的局部冷保存装置 ,将肾脏置于 0～ 4℃生理盐水中 ,8h后开放肾动、静脉使肾脏复灌 ,复灌前切除右侧肾脏。B组 :采用同样方法游离暴露肾脏后 ,不实施肾脏血流阻断和肾脏灌注 ,单纯低温保存 8h ,随后同法切除右肾。A、B组分别于肾脏复灌后 2h、2 4h抽取血液 2ml;肾脏复灌后 2 4h后切除左侧肾脏。结果 :A组 2h、2 4h血清Cr、Bun值均高于B组 ,差异有显著性 (P <0 .0 1) ;A组肾脏病理切片可见典型肾脏缺血再灌注损伤改变。结论 :大鼠自体原位肾移植缺血再灌注损伤模型手术操作简便 ,手术成功率高 ,充分模拟临床肾移植IRI特点 ,是研究肾脏移植缺血再灌注损伤的理想动物模型

The Foundation of Rat Orthotopic Kidney Auto-transplantation Ischemia Reperfusion Injury Models

Objective: To build a convenient rat model of orthotopic kidney autotransplantation and study the kidney ischemia reperfusion injury (IRI) after transplantation. Methods: 20 male Sprague Dawley rats were equally divided into model team and sham team. The rats were anesthetized with 1% pentobarbital sodium at a dose of 30 mg/kg.In the model team, the left kidney was flushed via kidney artery with 0～4 ℃ 0.9% NaCl. After sewing up the artery incision, the left kidney vein was clamped. Then the left kidney was subject to an 8 h cold storage in situ, followed by reestablishment of renal perfusion. Before the reperfusion, the right kidney was cut off. The sham team underwent 8 h hypothermal storage, and the right kidney was cut off at the end of 8 h cold storage. Blood samples were collected at 2 h,24 h after the kidney reperfusion for the BUN and Cr analysis. The left kidney was cut off at 24 h after the reperfusion and prepared for pathological examination. Results: BUN and Cr in the model team increased significantly compared with the sham team. And the pathological results of the model team showed a typical IRI. Conclusion: The rat orthotopic kidney auto transplantation model can be imitated by the clinical kidney transplantation procedure, and it shows a typical IRI. This model is a new and ideal animal model for the study of kidney IRI after transplantation.