荧光定量PCR检测HBV DNA与血清HBV 标志物和preS1相互关系研究

目的探讨荧光定量PCR法检测乙型肝炎病毒(HBV)DNA与HBV血清免疫学标志(HBVM)模式,preS1之间的相互关系及临床意义。方法采用实时荧光定量PCR法(FQ-PCR)检测801例血清的HBVDNA,用ELISA方法对其免疫学标志,preS1同时进行检测。结果HBVDNA的总检出率为67·0%,preS1的总检出率为74·9%。其中,在模式HBsAg( ),HBeAg( ),HBcAb( )中血清HBVDNA含量明显高于HBsAg( ),HBeAb( ),HcAb( )及HBsAg( ),HBcAb( )组。在HBsAg(-)模式中HBVDNA亦有检出。在HBsAg( ),HBeAb( ),HcAb( )组,HBVDNA的检出率为55·8%,而preS1的检出率为69·8%,两者有显著差异(P<0·01),其余HBVM模式,无显著差异(P>0·05)。结论HBVM和preS1提供了HBV感染的间接证据,荧光定量PCR法检测HBVDNA准确灵敏,是HBV感染及复制的直接证据,HBVM,preS1和HBVDNA的检测各有其独特的临床检测意义,在乙型肝炎的诊断治疗中均具有重要的临床指导价值。

Study on the relationship among quantitative HBVDNA tested by PCR, HBV M and preS1

Objective To investigative the relationship between HBV DNA tested by quantitative fluorescence PCR technique, hepatitis B virus markers(HBV M) and preS1. Methods HBV DNA was tested in 801 serum samples by real time fluorescence quantitative PCR(FQ-PCR). HBV M and preS1 were also tested by ELISA . Results The total serum HBV DNA and the preS1 positive rate was 67.0% and 74.9% respectively. In the group with positive HBsAg, HBeAg, HBcAb, the contents of HBV DNA were significantly greater than that of groups with positive HBsAg, HBeAb, HBcAb or HBsAg, HBcAb. HBV DNA was also tested positive in some of the serum with negative HBsAg The positive rate of HBV DNA was lower than that of preS1 in the group with positive HBsAg, HBeAb, HBcAb(P<0.01), but in the other groups, they had no significant difference(P>0.05). Conclusion HBV M and preS1 can give indirect proof of HBV infection. The quantitative fluorescence PCR determination of serum HBV DNA was correct and sensitive and could provide direct evidence of HBV infection and replication. They are of unique clinical significance, Therefore it is necessary to detect HBV M, preS1 and HBV DNA in the diagnosis and therapy of HBV hepatitis.