伤速康巴布膏对兔膝骨性关节炎模型关节液中SOD、NO水平变化影响的实验研究

目的：本实验选用伤速康巴布膏作为干预因素,观察在兔膝骨性关节炎模型用药后关节液中NO和SOD含量变化,从而探讨伤速康巴布膏治疗膝骨性关节炎疗效及其作用机理。方法：日本大耳白兔30只,雌雄不限,体重（1.85±0.15）kg,随机分为A组（模型对照组）、B组（伤速康巴布膏组）、C组（骨痛贴组）,每组10只。A、B、C 3组在＂Hulth造模法＂的基础上制作膝骨性关节炎模型。A组只以胶布固定,B组伤速康敷贴,C组骨痛贴敷贴胶布固定。于第2、4周,各组随机空气栓塞处死5只兔子,处死后用注射器抽取1mL生理盐水注入关节腔进行冲洗,4000r/min,取上清液,按试剂盒方法,检测关节液中超氧化物歧化酶（SOD）、一氧化氮（NO）的含量。结果：模型组关节液NO含量显著高于伤速康巴布膏组及骨痛贴组（P〈0.05）,伤速康巴布膏及骨痛贴可降低关节液NO水平,伤速康巴布膏组与骨痛贴组比较差异无统计学意义（P〉0.05）。模型组关节液SOD含量显著低于伤速康巴布膏组与骨痛贴组（P〈0.05）,伤速康巴布膏组与骨痛贴组比较差异无统计学意义（P〉0.05）。结论：伤速康巴布膏敷贴可对骨关节炎软骨退变起明显缓解作用;能显著降低兔关节液中N0含量以及升高关节液中SOD含量,并激发OA早期的关节软骨细胞增殖而治疗骨关节病,改善关节功能。

Effect of Shangsukang on the Levels of NO and SOD in Joint Fluid in Rabbit Knees with Osteoarthritis

Objective：This experiment aimed to investigate the effect of Shangsukang on rabbit knees with osteoarthritis and the mechanisms involved.Methods：Thirty healthy Japanese rabbits,without gender limits,weighing approximately（1.85±0.15）kg,were randomly divided into three groups as group A（model group）,group B（Shangsukang group）,and group C（Gutongtie group）,ten in each group.The model of knee osteoarthritis was made according to Hulth method.After modeling,rabbit knees in three groups were fixed respectively with tape,Shangsukang and Gutongtie for 4 weeks.In the 2nd and 4th weeks,5 rabbits of each group were sacrificed.The levels of SOD and NO in the knee joint fluid were detected.Results：No content was remarkably higher in model group than that in Shangsukang and Gutongtie groups（P0.05）,while SOD level was significantly lower in model group than that in Shangsukang and Gutongtie groups（P0.05）.There was no obvious difference in the levels of NO and SOD between Shangsukang group and Gutongtie group（P0.05）.Conclusion：The traditional Chinese medicine Shangsukang can delay the cartilage degradation in osteoarthritis knees,decrease the levels of NO and SOD,and stimulate proliferation of articular cartilage at the early stage of osteoarthritis.