HPLC法测定镇江橡胶膏中士的宁及马钱子碱的含量

目的:建立镇江橡胶膏中士的宁及马钱子碱含量测定方法.方法:采用HPLC法,色谱柱为Kromasil-C18 (4.6 mm×250 mm,5μm),流动相为A-B (11:89)A为乙腈,B由水-冰醋酸-三乙胺(230:2.4:0.3)组成],检测波长254 nm,流速1.0mL·min-1,柱温30C.结果:士的宁、马钱子碱分别在0.0885～1.327 μg(r=0.9997)、0.0522～0.782 μg(r=0.9960)范围内线性关系良好,平均回收率分别为99.6％、98.2％.结论:该方法准确、快速、重现性好,能有效控制本品质量.

Determination of Strychnine and Brucine in Zhenjiang Adhesive Plaster by HPLC

Objective:To establish a method for the determination of strychnine and brucine in the Zhenjiang Adhesive Plaster.Methods:The HPLC method was carried out on a column Kromasil-C18(4.6 mm×250 mm,5 μm) at the temperature of 30℃,the mobile phase was composed of A-B(11:89)(A for acetonitrile,B for water-acetic acid-triethylamine(230:2.4:0.3)),the flow rate was at 1.0 mL·min-1,the UV detection wavelength was at 254 nm.Results:The calibration curves of strychnine and brucine were in good linearities over the ranges of 0.0885～1.327 μg(r=0.9997) and 0.0522-0.782 μg(r=0.9960),respectively,and the average recoveries were 99.6% and 98.2%,respectively.Conclusion:This method is accurate,rapid,reproducible and suitable for the quality control of this preparation.