Cloning and characterization of Entamoeba histolytica antigens recognized by human secretory IgA antibodies |
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Authors: | Julio César Carrero Pavel Petrossian Emilio Acosta María Sánchez-Zerpa Librado Ortiz-Ortiz Juan Pedro Laclette |
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Institution: | (1) Department of Immunology, Instituto de Investigaciones Biomédicas, UNAM, A.P. 70228, 04510 México D.F., México e-mail: laclette@servidor.unam.mx Tel.: +525-622-3860; Fax: +525-622-3369, MX |
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Abstract: | To identify the Entamoeba histolytica antigens capable of inducing secretory IgA (sIgA) responses in humans, a cDNA library from the strain HM1:IMSS was immuno-screened
with saliva from patients with intestinal amebiasis or amebic liver abscess. Clones isolated with sIgA antibodies from patients
with intestinal amebiasis corresponded to the known serine-rich protein isoform, a 29 kDa cysteine-rich protein and 1-α elongation
factor. Clones corresponding to enolase, cyclophilin, ribosomal protein L23a, and an Hsp70 family protein were isolated with
sIgA from a patient with amebic liver abscess. A glutamic acid-rich peptide (EhGARP) positive with sIgA from a patient with
amebic liver abscess was also isolated; for EhGARP, no homologs were found in the protein databases. The antigens isolated
are potentially useful in the development of an oral vaccine or new diagnostic tools for amebiasis.
Received: 14 June 1999 / Accepted: 5 October 1999 |
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