Tri-lineage potential of intraoral tissue-derived mesenchymal stromal cells |
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Authors: | Birgit Lohberger Michael Payer Beate Rinner Heike Kaltenegger Elisabeth Wolf Katharina Schallmoser Dirk Strunk Eva Rohde Andrea Berghold Karin Pekovits Angelika Wildburger Andreas Leithner Reinhard Windhager Norbert Jakse |
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Affiliation: | 1. Biomedical Systems, Health & Environment Department, AIT Austrian Institute of Technology GmbH, Seibersdorf, Austria;2. Center for Medical Statistics, Informatics, and Intelligent Systems, Medical University of Vienna, Vienna, Austria;3. Department of Nuclear Medicine, Hannover Medical School, Hannover, Germany;4. Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine, and Centre for Systems Neuroscience, Hannover, Germany;5. Department of Medicinal Chemistry, University of Vienna, Vienna, Austria;6. Department of Clinical Pharmacology, Medical University of Vienna, Vienna, Austria |
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Abstract: | The purpose of this study was to analyse the potential of intraoral tissues as a source of mesenchymal stromal and progenitor cells (MSPCs) for usage in future cell-based therapy models.Cells were isolated from four different tissues harvested during oral surgery intervention: (1) bone explants from the posterior maxilla, (2) bone explants from the oblique line, (3) from the mandibular periosteum, and (4) from the dental pulp. Donor sites and tissues were evaluated in terms of their accessibility, donor-site morbidity and average time period until appearance of MSPC colonies. Cell characterization was performed by flow cytometry and evaluation of in vitro osteogenic, adipogenic and chondrogenic differentiation potential.Adherent cell colonies were isolated from tissues from all sites after 4–8 days. The cells showed characteristics of MSPCs, so they were expanded up to clinical scales and demonstrated multipotency. The lowest donor-site morbidity was observed in the posterior maxilla harvests, while the highest donor-site morbidity was associated with harvests from mandibular sites.All sites seem to be potential sources of mesenchymal stromal and progenitor cells for tissue engineering approaches. Therefore, harvest morbidity and patient acceptance should affect the choice of the appropriate site. |
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