MCMV感染对小鼠海马[Ca2+]i及线粒体膜电位的影响

目的:观察鼠巨细胞病毒(MCMV)感染对小鼠海马细胞内游离Ca2+浓度(犤Ca2+犦i)及线粒体膜电位的影响。方法:将BALB/C乳鼠同母鼠随机分为实验对照组(24只)和病毒组(35只),感染后56d应用荧光分光光度计和流式细胞仪分别检测两组小鼠海马的犤Ca2+犦i及线粒体膜电位。结果:病毒感染组小鼠海马犤Ca2+犦i增高及线粒体膜电位明显下降,与实验对照组比较差异有显著性(P值均<0.01)。结论:巨细胞病毒感染所引起的小鼠海马犤Ca2+犦i及线粒体膜电位的改变可能是其导致神经系统功能紊乱的机制之一。

Effect of [Ca~(2+)]i and neuronal mitochondria transmembrane potentials in hippocampus of mice cytome-galovirus infected mice

Objective To explore effect of [Ca2 ]i and neuronal mitochondria transmembrane potentials in hippocampus of mice cytomegalovirus(MCMV) infected mice. Methods Newborn mice and femal mice of BALB/C were randomly divided into two groups: the virus inoculated group and the control group. After 56 days, single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the intracellular free calcium level [Ca2 ]i in hippocampus were measured by means of fluorospectro- photometer and flow cytometry (FCM). Results Compared with the control group, the mitochondria transmembrane potentials was decreased (P< 0.01) and the intracellular free calcium level [Ca2 ]i was increased (P< 0.01) in inoculated group. Conclusion The dysfunction of [Ca2 ]i and mitochondria transmembrane potentials in hippocampus may play an important role in the CMV infected brain.