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MCMV感染对小鼠海马[Ca2+]i及线粒体膜电位的影响
引用本文:张英,闻良珍,程苾恒. MCMV感染对小鼠海马[Ca2+]i及线粒体膜电位的影响[J]. 实用医学杂志, 2006, 22(1): 11-12
作者姓名:张英  闻良珍  程苾恒
作者单位:430030,武汉市,华中科技大学同济医学院附属同济医院妇产科
摘    要:目的:观察鼠巨细胞病毒(MCMV)感染对小鼠海马细胞内游离Ca2+浓度(犤Ca2+犦i)及线粒体膜电位的影响。方法:将BALB/C乳鼠同母鼠随机分为实验对照组(24只)和病毒组(35只),感染后56d应用荧光分光光度计和流式细胞仪分别检测两组小鼠海马的犤Ca2+犦i及线粒体膜电位。结果:病毒感染组小鼠海马犤Ca2+犦i增高及线粒体膜电位明显下降,与实验对照组比较差异有显著性(P值均<0.01)。结论:巨细胞病毒感染所引起的小鼠海马犤Ca2+犦i及线粒体膜电位的改变可能是其导致神经系统功能紊乱的机制之一。

关 键 词:巨细胞病毒   感染   海马     线粒体膜电位  
收稿时间:2005-06-28
修稿时间:2005-06-28

Effect of [Ca~(2+)]i and neuronal mitochondria transmembrane potentials in hippocampus of mice cytome-galovirus infected mice
ZHANG Ying,WEN Liang-zhen,CHENG Bi-heng. Effect of [Ca~(2+)]i and neuronal mitochondria transmembrane potentials in hippocampus of mice cytome-galovirus infected mice[J]. The Journal of Practical Medicine, 2006, 22(1): 11-12
Authors:ZHANG Ying  WEN Liang-zhen  CHENG Bi-heng
Affiliation:Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Abstract:Objective To explore effect of [Ca2 ]i and neuronal mitochondria transmembrane potentials in hippocampus of mice cytomegalovirus(MCMV) infected mice. Methods Newborn mice and femal mice of BALB/C were randomly divided into two groups: the virus inoculated group and the control group. After 56 days, single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the intracellular free calcium level [Ca2 ]i in hippocampus were measured by means of fluorospectro- photometer and flow cytometry (FCM). Results Compared with the control group, the mitochondria transmembrane potentials was decreased (P< 0.01) and the intracellular free calcium level [Ca2 ]i was increased (P< 0.01) in inoculated group. Conclusion The dysfunction of [Ca2 ]i and mitochondria transmembrane potentials in hippocampus may play an important role in the CMV infected brain.
Keywords:Cytomegalovirus Infection Hippocampus Calcium Mitochondria transmembrane potential
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