| 血管内皮细胞生长因子表位肽体外抗肿瘤作用研究 |
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| 引用本文： | 赵鑫,王宏,向军俭,朱中松,宋其芳,邓宁. 血管内皮细胞生长因子表位肽体外抗肿瘤作用研究[J]. 中国免疫学杂志, 2010, 26(5). DOI: 10.3969/j.issn.1000-484X.2010.05.005 |
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| 作者姓名： | 赵鑫 王宏 向军俭 朱中松 宋其芳 邓宁 |
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| 作者单位： | 暨南大学生命科学技术学院抗体工程研究中心,广州,510632 |
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| 基金项目： | 广州市科技攻关重点项目资助 |
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| 摘 要： | 目的:研究筛选血管内皮细胞生长因子(VEGF)不同表位肽抗肿瘤及抗血管新生效用。方法:构建4种不同的pCANTAB5-E/VEGF表位肽载体,将VEGF表位肽通过噬菌体展示技术展示于噬菌体表面,MTT法检测VEGF噬菌体表位肽对黑色素瘤细胞(B16)、人脐静脉血管内皮细胞(HUVEC)增殖的影响,Transwell小室检测其对B16F10细胞迁移的影响,ECMgel检测其对HUVEC成管的影响。结果:筛选出VEGF的4种(VSB、VNB、VEB、VTB)表位肽中,VNB对HUVEC细胞增殖的抑制率达到64.8%,VEB、VTB对B16的增殖抑制率分别为75.7%和66.5%。4株表位肽对高转移细胞B16F10迁移抑制率均超过80%。结论:筛选得到的4株VEGF表位肽对黑色素瘤细胞、血管内皮细胞的增殖、迁移和成管有抑制作用,通过分析其表位区段后可制备成小分子多肽,为今后抗肿瘤多肽药物和疫苗的研发奠定基础。
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| 关 键 词： | 表位肽 抗肿瘤 抗血管新生 |
Anti-tumor effect of epitopic peptides of vascular endothelial growth factor in vitro |
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| ZHAO Xin,WANG Hong,XIANG Jun-Jian,ZHU Zhong-Song,SONG Qi-Fang,DENG Ning. Anti-tumor effect of epitopic peptides of vascular endothelial growth factor in vitro[J]. Chinese Journal of Immunology, 2010, 26(5). DOI: 10.3969/j.issn.1000-484X.2010.05.005 |
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| Authors: | ZHAO Xin WANG Hong XIANG Jun-Jian ZHU Zhong-Song SONG Qi-Fang DENG Ning |
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| Affiliation: | ZHAO Xin,WANG Hong,XIANG Jun-Jian,ZHU Zhong-Song,SONG Qi-Fang,DENG Ning.Antibody Engineering Center of College of Life Science , Technology,Jinan University,Guangzhou 510632,China |
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| Abstract: | Objective:This study was to get several peptides of vascular endothelial growth factor(VEGF) which were designed by computer simulation and the specific anti-tumor peptides were selected to use in tumor inhibition.Methods:The peptides were designed and displayed in the surface of phages by using phage display system.Anti-tumor and anti-vascular endothelial cell effects of the peptides on B16 and human umbilical vein endothelial cells(HUVEC) were analyzed with MTT assay.Tumor cell migration assay was applied... |
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| Keywords: | VEGF |
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