E—cad和VEGF在妊娠滋养细胞疾病中的表达及意义

目的观察E—cad和VEGF在妊娠滋养细胞疾病中的表达变化及意义，为妊娠滋养细胞疾病早期诊断、预后判断提供理论依据。方法收集滋养细胞疾病标本50例，其中葡萄胎10例（均为完全性葡萄胎），侵蚀性葡萄胎20例，绒毛膜癌20例，所有病例均经病理确诊。正常早孕绒毛标本10例为对照组。应用免疫组织化学技术检测E-cad和VEGF在早孕绒毛、葡萄胎、侵蚀性葡萄胎、绒毛膜癌组织中的表达情况。结果E—cad在正常早孕绒毛、葡萄胎、侵蚀性葡萄胎和绒癌中的表达逐渐降低，分别为3．521，3．1045，0．9655，0．7682，各组差异有统计学意义（P〈0．05）；VEGF在正常早孕绒毛、葡萄胎、侵蚀性葡萄胎和绒癌中的表达逐渐升高，分别为0．4219，0．7521，0．9611，1.1420，各组差异有统计学意义（P〈0.05）。结论①E-cad在滋养细胞肿瘤和葡萄胎中的表达均低于正常绒毛组织，E-cad表达减弱与GTT恶性进展有关，对于良、恶性葡萄胎鉴别有重要意义。②E—cad和VEGF的表达变化，使滋养细胞粘附异常，侵蚀性增强，是滋养细胞疾病发生的重要途径之一。

Expression and Function of VEGF and E-cad in Gestational Trophoblastic Disease

Objective To study the expression of E-cadherin （E-cad ） and vascular endothelial growth factor （VEGF） in gestational trophoblastic disease and provide theoretical basis for its early diagnosis and treatment. Methods Ten cases of hydatidiform mole ,20 cases of invasive hydatidiform mole and 20 cases of choriocarcinomas were selected. Ten cases of normal villi tissues served as the control group. Expression of E-cad and VEGF in normal chorion of ear- ly gestation, hydatidiform mole, invasive hydatidiform mole and choriocarcinoma were detetected with immunohistochemistry method and image analysis technique. Results ①The expression of E-cad in normal villi, hydatidiform mole, invasive hydatidiform mole and choriocarcinomas was 3. 521,3. 1045,0. 9655,0. 7682,gradually declined with the progression of gestational trophoblastic disease （ P 〈 0.05 ）. ②The expression of VEGF in normal villi, hydatidiform mole, invasive hydatidiform mole and choriocareinomas was 0. 4219,0. 7521,0. 9611,1. 1420, significantly elevated gradually with the progression of gestational trophoblastic disease （ P 〈 0.05 ）. Conclusions ①The lower expression of E-cad is related to the progression of gestational trophoblastic disease and helpful to distinguish choriocarcinomas from hydatidiform mole. The overexpression of VEGF is associated with the deterioration of GTT.②The expression changes of E-cad and VEGF may play an important role in the development of gestational trophoblastic tumor.