口腔鳞癌中Bax,Bcl-2和Survivin 蛋白表达与预后相关性研究

目的:研究凋亡相关蛋白Bax,Bcl-2及Survivin的表达与口腔鳞癌预后的相关性,确定预后判断的有效标志物。方法:采用免疫组织化学方法,检测110例口腔鳞癌患者术后石蜡切片组织中Bax、Bcl-2及Survivin蛋白的表达。通过目标蛋白阳性染色细胞比例和阳性细胞中蛋白的染色强度判定各种蛋白的表达水平;应用SAS 9.0软件中的Kaplan-Meier及Cox回归分析Bax、Bcl-2及Survivin蛋白表达与口腔鳞癌预后的相关性。结果:110例标本检测和统计分析结果表明,Bax、Bcl-2和Survivin蛋白单独表达水平与口腔鳞癌术后生存时间并无显著的相关性;Bcl-2/Bax的比值与口腔鳞癌的术后生存时间呈显著相关(P<0.05)。结论:口腔鳞癌患者组织标本中的Bcl-2/Bax值是影响预后的重要指标,提示该指标在临床上可作为口腔鳞癌患者预后判断的有效标志物。

Bax,Bcl-2 and Survivin Expression in Oral Squamous Cell Carcinoma: Significance to Prognosis

Objective: To investigate the correlation between expression of Bax,Bcl-2,Survivin proteins in oral squamous cell carcinoma and their significance to prognosis.Methods: The expression of Bax,Bcl-2 and Survivin were evaluated by immunohistochemical method in formalin fixed paraffin embedded specimens from 110 patients with oral squmaous cell carcinoma,which were followed-up at least 5 years after operation.Every section was scored according to the fraction of stained tumor cells and the staining intensity.The Kaplan-Meier test and Cox proportional hazards regression analysis were performed to identify the relationship between the proteins expression level and prognosis of the patients.Results: The ratio of Bcl-2/Bax expression was proved to be an independent prognostic parameter in patients with oral squmaous cell carcinoma.But Bax or Bcl-2 or Survivin expression alone showed no significance to prognosis.Conclusion: The ratio of Bcl-2/Bax expression was an independent prognostic parameter of oral squmaous cell carcinoma patients.