三氯醋酸与Carnoy液对兔血管壁作用的实验研究

目的：为探讨三氯醋酸与Ｃａｒｎｏｙ液局部应用时对血管壁可能造成的损伤和组织的修复情况。方法：采用动物实验的方法，分别以３５％三氯醋酸、２０％三氯醋酸、Ｃａｒｎｏｙ液及生理盐水涂布于兔血管壁４分钟，术后第１天，第１、４、８周切取标本行组织学、酶组织化学及透射电镜检查。结果：药液作用后第１天血管壁各层乳酸脱氢酶活性均丧失或减弱，但组织学检查未发现结构破坏。术后第１周，血管壁胶原纤维和平滑肌细胞坏死，内皮细胞坏死脱落。第４～８周，血管内皮及平滑肌逐渐恢复正常。结论：实验中使用的药液直接作用时间不超过４分钟时，受损血管可被修复

STUDY OF EFFECTS OF TRICHLOROACETIC ACID AND CARNOY'S SOLUTION ON THE VESSEL WALLS OF RABBIT

Objective In order to study of effects of trichloroacetic acid and Carony's solution on the vessel walls.Method Sixteen rabbits were used and the vessel walls were spread with 35% TCA、20% TCA、Carony's solution and physiological salt solution respectively for 4 minutes. The animal were separately sacrified at 1 day and 1, 4, 8 weeks postoperatively. The specimens were made for histopathological、enzyme histochemical and transimission electron microscope observation.Results In the experimental animals at 1 day, no destruction of vessel wall was observed, but the stain of lactate dehydrogenase (LDH) were negative, at 1 week collagen fibrils and smooth muscle cells were necrosed and endothelial cells were also necrosed and peeled. At 4 weeks, the regenerative cells were obvious, and at 8 weeks their constructure and stain of LDH were almost normal. Conclusion The injuried vessel will be grdually repaired when they are contacted with TCA and Carnoy's solution within 4 minutes.