| 血液分离耐甲氧西林金黄色葡萄球菌的脉冲场凝胶电泳分析 |
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| 引用本文： | 陈荣,陈刚,沈定霞,王欢,叶丽艳. 血液分离耐甲氧西林金黄色葡萄球菌的脉冲场凝胶电泳分析[J]. 军医进修学院学报, 2010, 31(6): 560-561,568 |
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| 作者姓名： | 陈荣 陈刚 沈定霞 王欢 叶丽艳 |
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| 作者单位： | 解放军总医院,微生物科,北京,100853 |
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| 基金项目： | 军队“十一·五”项目(06MA294)~~ |
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| 摘 要： | 目的了解我院患者血液分离耐甲氧西林金黄色葡萄球菌（MRSA）的分子流行病学特征。方法收集2006年1月-2008年12月我院患者血液分离耐甲氧西林金黄色葡萄球菌22株,采用脉冲场凝胶电泳技术进行分子分型。结果耐甲氧西林金黄色葡萄球菌可分为A-F共6个型别,其中A型13株、B型4株、C型2株,D、E、F型各1株;A型还可分为A1-A6共6个亚型,B型也可分为2个亚型。结论我院患者血液分离的耐甲氧西林金黄色葡萄球菌菌株在分子水平上有较近的亲缘关系。
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| 关 键 词： | 葡萄球菌,金黄色 血传病原体 细菌分型技术 电泳,凝胶,脉冲场 |
Pulse-field gel electrophoresis of methicillin-resistant Staphylococcus aureus |
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| CHEN Rong,CHEN Gang,SHEN Ding-xia,WANG Huan,YE Li-yan. Pulse-field gel electrophoresis of methicillin-resistant Staphylococcus aureus[J]. Academic Journal of Pla Postgraduate Medical School, 2010, 31(6): 560-561,568 |
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| Authors: | CHEN Rong CHEN Gang SHEN Ding-xia WANG Huan YE Li-yan |
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| Affiliation: | CHEN Rong,CHEN Gang,SHEN Ding-xia,WANG Huan,YE Li-yan Department of Microbiology,Chinese PLA General Hospital,Beijing 100853,China |
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| Abstract: | Objective To investigate the molecular feature and epidemiology of methicillin resistant Staphylococcus aureus(MRSA). Methods Twenty-two MRSA strains isolated from blood of patients in our hospital from January 2006 to December 2008 were collected.Their genotypes were determined by pulse field gel electrophoresis(PFGE).Results The finger printing of PFGE showed 6 genotypes(A-F)of MRSA strains including 13 of A,4 of B,2 of C,and 1 of D,E and F each.Among them,A and B were the major genotypes and could be fur... |
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| Keywords: | Staphylococcus Aureus Blood-Borne Pathogens Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field |
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