| 蛋白印迹法人血清DNA结合蛋白的测定 |
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| 引用本文： | 许道松,马涧泉.蛋白印迹法人血清DNA结合蛋白的测定[J].中山大学学报(医学科学版),1991(4). |
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| 作者姓名： | 许道松 马涧泉 |
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| 作者单位： | 中山医科大学生物化学教研室，中山医科大学生物化学教研室 硕士 |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 利用蛋白印迹法(Western blot)原理建立了检测人血清DNA结合蛋白的一种较特异性方法,发现在成人血清中有7 种Lambda DNA结合蛋白,分子量为:90kDa、67kDa、43kDa、30kDa、24kDa、22kDa和17kDa。在婴儿血清中没有30kDa、24kDa和22kDa这3种LambdaDNA结合蛋白。在人的血清中有二种小牛胸腺DNA结合蛋白,分子量为:43kDa和17kDa。血清中有四种与正常成人血浆DNA结合的蛋白质,分子量分别为:67kDa、43kDa、30kDa和24kDa,其中30kDa的这种蛋白质与正常成人血浆DNA的结合力较弱,并在一些正常个体中未见到此带。
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| 关 键 词： | 蛋白印迹法 人血清 DNA结合蛋白 |
DETECT SERUM DNA BINDING PROTEINS BY WESTERN BLOT |
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| Xu Daosong Ma Jianquan.DETECT SERUM DNA BINDING PROTEINS BY WESTERN BLOT[J].Journal of Sun Yatsen University(Medical Sciences),1991(4). |
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| Authors: | Xu Daosong Ma Jianquan |
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| Institution: | Department of Biochemistry |
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| Abstract: | A method based on Western blot was designed and used for detecting DNA-binding proteins in human sera. Several DNA-binding proteins in human sera were discovered. There are seven kinds of Lambda phage DNA binding proteins in human sera. Their molecular weights are as follows: 90kDa, 67kDa, 43kDa, 30kDa, 24kDa, 22kDa and 17kDa. Three kinds of DNA-binding proteins with MW 30kDa 24kDa and 22 kDa were not detected in baby sera. Two kinds of calf thymus DNA-binding proteins were detected in human sera. Their molecular weights are 43kDa and 17kDa. Four kinds of human plasma DNA binding paoteins can be detected in human sera. The molecular weights are G7kDa, 43kDa, 30kDa and 24kDa. The proteins with MW 30kDa has a weak binding to human blood plasma DNA, and in some healthy individuals this protein is absent. |
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| Keywords: | Western blotn Human serum DNA-binding proteins |
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