混合接种丛枝菌根真菌对木香幼苗生长及化学成分的影响

目的:探讨丛枝菌根(AM)真菌混合接种的协同作用对木香幼苗的生长、生理生化特性、根生物量及萜类成分积累的影响,为优势互补效应菌根真菌的组合配置应用提供参考。方法:采用室温盆栽接种实验,通过不同菌株的结合,以不接种为对照,测定不同AM真菌结合接种处理对木香幼苗的根系菌根侵染率、植株生长与生理生化特性、根生物量及木香烃内酯、去氢木香内酯含量的影响。结果:供试AM真菌能与木香根系形成良好菌根共生体,菌根共生体系的形成后可增加木香叶片的叶绿素含量,提高过氧化氢酶(CAT),过氧化物酶(POD),超氧化物歧化酶(SOD)的酶活性,降低丙二醛(MDA)含量。与空白CK组相比,AM真菌处理组能显著促进木香烃内酯、去氢木香内酯含量的积累,木香幼苗共生栽培过程中显著促进其代谢产物木香烃内酯、去氢木香内酯向根中累积,间接提高了木香的入药品质和木香内酯产量。结论:接种外源性AM真菌可提高木香根系菌根生活力,增加对营养物质的吸收,促进木香营养生长。在人工栽培中,S2,S4,S5混合接种处理组的菌根效应最佳,木香生长和药用品质达到最优,为木香菌根生物技术的应用推广提供了技术支撑。

Effect of Mixed Inoculation of Arbuscular Mycorrhizal on Growth and Chemical Composition of Aucklandia lappa Seedlings

Objective:To explore the synergistic effect of arbuscular mycorrhizal(AM)fungi mixed inoculation on the growth,physiological and biochemical characteristics,root biomass and terpenoid component accumulation of Aucklandia lappa seedlings,so as to provide a reference for the combination and application of the dominant complementary effect mycorrhizal fungi.Method:The effect of different AM fungi combined with inoculation on the root mycorrhizal infection rate,plant growth,physiological and biochemical characteristics,root biomass,costunolide and dehydrocostus lactone of A.lappa seedlings were determined by pot inoculation at room temperature.Result:It was found that AM fungi could form good mycorrhizal symbiosis with the roots of A.lappa.The formation of mycorrhizal symbiosis system could increase the chlorophyll content of A.lappa leaves,increase the activities of catalase(CAT),peroxidase(POD),superoxide dismutase(SOD),reduce the content of malondialdehyde(MDA),and promote photosynthesis of A.lappa.Compared with CK group,AM fungus treatment could significantly promote the accumulation of costunolide and dehydrocostus lactone,and the accumulation of its metabolites,costunolide and dehydrocostus lactone,into roots during the symbiotic cultivation of A.lappa seedlings,indirectly improving the quality of medicines and yield of alantolactone.Conclusion:Inoculation of AM fungi can improve the root mycorrhizal viability,increase the absorption of nutrients and promote the growth of woody incense.The mixed inoculation treatment of S2,S4 and S5 had the best mycorrhizal effect in artificial cultivation,and the growth and medicinal quality of A.lappa were the best,which provided technical support for the application and popularization of A.lappa mycorrhizal biotechnology.