聚普瑞锌对大鼠急性胃黏膜损伤的修复作用

目的：探讨聚普瑞锌促进大鼠酒精性急性胃黏膜损伤的修复作用机制及与前列腺素E2(prostaglandin E2，PGE2)、表皮生长因子(epidermal growth factor，EGF)的关系。方法：30只SD大鼠随机分为空白组、模型组、治疗组，每组10只。空白组给予常规饲养，其余两组给予无水乙醇1 mL制造急性糜烂性胃炎模型。1 h后治疗组予1 mL聚普瑞锌溶液(按聚普瑞锌100 mg/kg体重计算)，并连续予聚普瑞锌灌胃3 d，每天1次；模型组则给予1 mL蒸馏水灌胃，连续3 d，每天1次。第3天灌胃前各组大鼠均禁食24 h，灌胃2 h后腹腔取血5 mL，离心取血清置EP管中，–80 ℃冰箱保存待测EGF和PGE2水平，并取大鼠胃行大体形态及病理组织观察。结果：治疗组大鼠胃黏膜大体及病理组织观察均较模型组愈合好。与空白组比较， 模型组、治疗组血清中PGE2和EGF含量明显降低，差异有统计学意义(P<0.05)，治疗组与模型组PGE2和EGF含量比较，差异无统计学意义(P>0.05)。结论：聚普瑞锌能促进急性胃黏膜损伤的修复，但其作用机制不受PGE2和EGF的调节。

Protective effect of polaprezinc on acute gastric mucosal injury in rats

Objective: To explore the role of prostaglandin E2 (PGE2) and epidermal growth factor (EGF) in the protective effect of polaprezinc on acute gastric mucosal damage. Methods: A total of 30 male SD rats were randomly divided into 3 groups as follows: A blank group, a model group, and a polaprezinc group. The blank group was fed normally. As for the model group and the polaprezinc group, the rats were given 1 mL ethanol (intragastrical administration) first, then they were treated with vehicle (1 mL distilled water) or polaprezinc treatment (100 mg/kg) 1 h later. On the 3rd day, rats in each group were fasting for 24 h before gastric administration. After 2 h of gastric administration, 5 mL of intraperitoneal blood was collected, centrifuged and stored at –80 ℃ for the detection of EGF and PGE2. Gastric tissue was collected for anatomic and pathological assessment. Results: Polaprezinc reduced gastric mucosa injury in the polaprezinc group compared to the model group. Compared with the blank group, the levels of PGE2 and EGF in blood serum were significantly decreased in the model group and the polaprezinc group (P<0.05), while there was no significant difference between the model group and the polaprezinc group (P>0.05). Conclusion: Polaprezinc can provide effective protection for acute mucosal injury and the underlying mechanism is not directly related to PGE2 and EGF.