桃红四物颗粒对寒凝血瘀痛经大鼠的治疗作用及机制

目的:观察桃红四物颗粒对寒凝血瘀痛经大鼠的干预作用。方法:健康SD雌性大鼠60只,随机分为6组,分别为正常组、模型组、桃红四物颗粒高、中、低剂量组(18,9,4.5 g·kg^-1)和妇科千金片组(0.08 g·kg^-1),每天灌胃补佳乐(0.35 mg·kg^-1)后冰水浴8 min,连续10 d,于第11天腹腔注射缩宫素(2U/只),建立寒凝血瘀型痛经大鼠模型。观察大鼠毛发色泽,精神状况,大鼠出现寒颤,弓背竖毛,耳尾爪苍白,反应迟钝,大便稀溏,小便色清等症状,注射完缩宫素后出现扭体反应即寒凝血瘀型痛经大鼠造模成功。测量桃红四物颗粒对造模成功大鼠血液流变学的影响,酶联免疫吸附测定(ELISA)检测大鼠血清中6-酮前列环素F1α(6-keto-PGF1α),血栓素B2(TXB2)和子宫组织中前列腺素F2α(PGF2α),前列腺素E2(PGE2)水平,用激光共聚焦显微镜扫描并检测子宫组织Ca^2+。结果:与正常组比较,模型组痛经大鼠的扭体次数明显升高(P<0.05,P<0.01),与模型组比较,桃红四物颗粒各剂量组均使痛经大鼠的扭体次数明显降低(P<0.05,P<0.01);与正常组比较,模型组全血黏度及血浆黏度明显升高(P<0.05,P<0.01),与模型组比较,桃红四物颗粒高、中、低剂量组可明显降低全血黏度及血浆黏度(P<0.05,P<0.01);与正常组比较,模型组血清中TXB2含量升高,6-keto-PGF1α含量降低,子宫中Ca^2+,PGF2α含量升高,PGE2含量降低,与模型组比较,桃红四物颗粒能显著降低血清中TXB2含量,显著升高血清中6-keto-PGF1α含量,显著降低子宫中Ca^2+,PGF2α含量,显著升高子宫中PGE2含量(P<0.05,P<0.01)。结论:桃红四物颗粒可以明显改善寒凝血瘀痛经大鼠症状,其作用机制可能与大鼠血清中6-keto-PGF1α,TXB2和子宫组织中PGF2α,PGE2以及Ca^2+含量有关。

Therapeutic Effect and Mechanism of Taohong Siwu Granule on Rats with Dysmenorrhea Due to Cold Coagulation and Blood Stasis

Objective:To observe the effect of Taohong Siwu granule on dysmenorrhea rats.Method:Forty-eight healthy SD female rats were randomly divided into six groups:control group,model group,Taohong Siwu granule groups(18,9,4.5 g·kg^-1)and Fuke Qianjin tablet group(0.08 g·kg^-1).The rats were given Bugaorer(0.35 mg·kg^-1)every day and ice-water bath for 8 minutes for 10 consecutive days.Oxytocin was injected intraperitoneally on the 11th day.The writhing reaction of rats was observed,and the effect of Taohong Siwu granules on hemorheology was measured.The levels of 6-ketoprostacyclin F1(α6-keto-PGF1α),thromboxane B2(TXB2),prostaglandinF2α(PGF2α),PGE2 of rats were detected by enzyme-linked immunosorbent assay(ELISA).Ca^2+in uterine tissues was scanned by laser confocal microscopy.Result:Each dose of Taohong Siwu granule group reduced the number of writhing,the time of writhing and the incidence of writhing in dysmenorrhea rats,which were significantly lower than those in model group(P<0.05,P<0.01).Compared with the model group,high,medium and low-dose Taohong Siwu granules groups could reduce blood viscosity and plasma viscosity,and Taohong Siwu granules can significantly reduce the content of TXB2,Ca^2+,PGF2α,and significantly increase the content of 6-keto-PGF1α,PGE2(P<0.05,P<0.01).Conclusion:Taohong Siwu granule can significantly alleviate dysmenorrhea symptoms in rats.The mechanism may be correlated with the contents of 6-keto-PGF1α,TXB2 and PGF2α,PGE2 and Ca^2+.