斑蝥酸钠协同雷公藤甲素诱导肝癌细胞株7721凋亡

目的：探讨斑蝥酸钠协同雷公藤甲素对人肝癌7721细胞生长的影响及机制。方法：采用系列浓度的斑蝥酸钠与雷公藤甲素单独或联合作用于人肝癌7721细胞，应用MTT法检测它们对人肝癌7721细胞生长抑制率的影响，荧光倒置显微镜法观察细胞形态的变化，流式细胞术检测细胞凋亡率，Western印迹法分别测定各组用药对人肝癌7721细胞中NF-κB p65和caspase-3表达的影响。结果：斑蝥酸钠和雷公藤甲素单独用药均可抑制人肝癌7721细胞的生长(P<0.05)，且呈剂量和时间依赖性。两药联合作用对人肝癌7721细胞生长抑制率和凋亡率均大于单独用药(P<0.05) ，并且两药联用可以显著下调NF-κB p65表达(P<0.05)，显著上调caspase-3的表达(P<0.05)。结论：斑蝥酸钠和雷公藤甲素均可抑制人肝癌7721细胞的生长，通过影响凋亡相关蛋白的表达诱导细胞凋亡；斑蝥酸钠可协同雷公藤甲素上调caspase-3的表达并下调NF-κB p65表达，联合用药效应明显。

Combination of triptolide with sodium cantharidinate synergistically enhances apoptosis on#br#hepatoma cell line 7721

Objective: To determine the combined cytotoxic effect and the molecular basis of triptolide and sodium cantharidinate on hepatoma cell line 7721.Methods: After treating the hepatoma cell line 7721 with triptolide(9, 18, or 36 μg/mL) and/or sodium cantharidinate (2, 5, or 10 μg/mL), cell viability assay and apoptosis were examined by MTT and flocytometry, respectively. The protein levels of caspase 3 and nuclear factor κB were analyzed by Western blot.Results: Viability of hepatoma cell line 7721 was inhibited by either the therapy of triptolide and/or sodium cantharidinate (P<0.05) in a time- and dose-dependent manner. The combined effects of both drugs were better than those of the single drug (P<0.05). The combined therapy down-regulated the expression of NF-κB p65 (P<0.05) while up-regulated the expression of caspase-3 (P<0.05).Conclusion: Triptolide and sodium cantharidinate exert a synergistic toxic effect on hepatoma cell line 7721, which is related to increasing capase-3 activity and suppression of NF- κB.