氢气对心搏骤停兔心肌糖代谢的PET显像的影响

目的：通过18F-氟-2-脱氧葡萄糖(18F-fluroro-2-deoxyglucose，18F-FDG)正电子发射断层成像(positron emission tomography，PET)了解心搏骤停兔心肌糖代谢的变化规律及氢气干预对其影响。 方法：雄性新西兰大白兔15只，分为3组：氢气组6只，对照组6只，假手术组3只。通过静脉注射氯化钾诱导心搏骤停兔模型，在心搏骤停5 min后开始行心肺复苏。氢气组与对照组开始复苏时分别予以4%氢气+96%氧气和纯氧吸入，持续30 min，假手术组麻醉、气管插管后予以肾上腺素注射+氯化钾缓慢注射，但未诱导心搏骤停。记录各组动物基础及复苏后30 min生命体征和复苏相关参数。3组均于基础状态及复苏后2和24 h利用PET测定左心室心肌18F-FDG最大标准化摄取值(maximum standardizeduptake value，SUVmax)。 结果：3组基础体重、生命体征及行PET检查前血糖水平差异均无统计学意义(均P>0.05)。假手术组3次左心室18F-FDG SUVmax值无显著变化。氢气组及对照组复苏后2 h左心室18F-FDG SUVmax较基础值明显升高(分别为1.89±0.47 vs 3.47±1.24及1.90±0.36 vs 4.26±0.80，P<0.01)，与对照组相比，氢气组升高幅度较低，24 h后均明显回降(氢气组 2.02±0.64，对照组2.07±0.61)，与基础值差异无统计学意义(P>0.05)。 结论：心搏骤停复苏后兔心肌糖代谢存在明显增强过程，氢气干预可降低糖代谢增强的程度。

Application of positron emission tomography in detection of myocardial glucose metabolism in rabbitcardiac arrest models

Objective: To investigate the changes of myocardial glucose metabolism in rabbit cardiac arrest models and the eff ect of hydrogen intervention by 18F-fl uroro-2-deoxyglucose (18F-FDG) positronemission tomography (PET) imaging.Methods: Fifteen male New Zealand white rabbits were randomly divided into a hydrogen group(n=6), a control group (n=6) and a sham group (n=3). Cardiac arrest (CA) was induced byintravenous injection of potassium chloride. Conventional cardiopulmonary resuscitation (CPR)was initiated after five-minutes CA. The hydrogen group and the control group were mechanicallyventilated into mixed gas with 4% hydrogen+96% oxygen and pure oxygen, respectively, for 30minutes after CPR. Rats in the sham group was performed the same surgical procedure and wasinjected adrenaline and potassium chloride but did not induce CA. The vital signs at basic stateand 30 min after return of spontaneous circulation (ROSC) were recorded in each group. Theparameters of CPR were recorded in two CA groups. Myocardial glucose metabolism was assessedby positron emission tomography (PET) at basic state, 2 h and 24 h after ROSC. The maximumstandardized uptake value (SUVmax) of 18F-FDG was measured.Results: There were no significant differences in the basal body weight and vital signs among thethree groups. There was no significant difference in the blood glucose level before PET examination.The 18F-FDG SUVmax in the sham group at three time points was not significantly changed. In thehydrogen group and the control group, the 18F-FDG SUVmax at 2 h after ROSC were significantlyhigher than the basic level (1.89±0.47 vs 3.47±1.24 and 1.90±0.36 vs 4.26±0.80, respectively).Compared with the control group, the 18F-FDG SUVmax in the hydrogen group was lower at thepoint at 2 h after ROSC. The 18F-FDG SUVmax in the 2 CA group were down to the basic level at24 h after ROSC (hydrogen group 2.02±0.64, control group 2.07±0.61).Conclusion: Myocardial glucose metabolism in CA rabbits was increased significantly after ROSC,and hydrogen intervention can reduce the degree of glucose metabolism.