丝氨酸/苏氨酸蛋白激酶激活的长链非编码RNA对甲状腺癌细胞凋亡和自噬行为的影响

目的：探讨丝氨酸/苏氨酸蛋白激酶(serine/threonine-protein kinase B-Raf，BRAF)激活的长链非编码 RNA(BRAF-activated long-chain non-coding RNA，lncRNA -BANCR)对甲状腺癌细胞凋亡和自噬行为的影响及其机制。 方法：RT-PCR检测lncRNA -BANCR在甲状腺癌组织和癌旁正常甲状腺组织中的表达情况；分析lncRNA-BANCR和甲 状腺癌患者的临床病理资料之间的关联；采用细胞计数试剂盒(cell counting kit-8，CCK-8)检测lncRNA-BANCR对甲状 腺癌细胞增殖能力的影响；流式细胞术检测lncRNA-BANCR对甲状腺癌细胞凋亡行为的影响；Transwell侵袭实验检测 lncRNA-BANCR对甲状腺癌细胞侵袭能力的影响，Wsetern印迹检测抑制lncRNA -BANCR的表达后自噬蛋白LC3-I和LC3- II表达的变化情况。结果：与正常甲状腺组织相比，甲状腺癌组织中lncRNA-BANCR表达水平较高(P<0.05)；lncRNABANCR 的表达与甲状腺癌的病理分期和淋巴结转移情况有关，分期越高，lncRNA -BANCR在甲状腺癌组织中表达 越高(P<0.05)；抑制lncRNA-BANCR的表达后，在一定程度上可抑制甲状腺癌细胞的增殖和侵袭能力(均P<0.05)；同 时可使甲状腺癌细胞的凋亡行为得到一定的促进(P<0.05)；自噬蛋白LC3-I和LC3-II的表达水平相应增高(均P<0.05)。 结论：lncRNA-BANCR的表达通过影响甲状腺癌细胞的自噬行为而对其增殖、侵袭及凋亡行为产生影响。

Effects of serine/threonine-protein kinase B-Raf-activated long-chain non-coding RNA on apoptosis and autophagy in thyroid carcinoma cells

Objective: To investigate the effect of serine/threonine-protein kinase B-Raf (BRAF)-activated long-chain non-coding RNA (lncRNA-BANCR) on apoptosis and autophagy in thyroid carcinoma cells and the underlying mechanisms. Methods: RT-PCR was used to detect the expression of lncRNA-BANCR in thyroid carcinoma and normal thyroid tissues. The association between lncRNA-BANCR and clinicopathological data was analyzed in patients with thyroid cancer. Cell counting kit-8 (CCK-8) was used to detect the effect of lncRNA-BANCR on the proliferation of thyroid cancer cells. The effect of lncRNA-BANCR on the apoptosis of thyroid carcinoma cells was detected by flow cytometry. Transwell invasion assay was used to detect the effect of lncRNA-BANCR on the invasive ability of thyroid cancer cells. Western blot was used to detect the changes of autophagy proteins LC3-I and LC3-II after the lncRNA-BANCR expression was suppressed. Results: Compared with normal thyroid tissues, the expression level of lncRNA-BANCR in thyroid carcinoma tissues was elevated (P<0.05). The expression of lncRNA-BANCR was positively related to the pathological stage of thyroid carcinoma and the lymph node metastasis. Inhibition of lncRNA-BANCR expression attenuated the proliferation and invasion ability of thyroid cancer cells (both P<0.05); but the apoptosis was enhanced (P<0.05); the expression levels of autophagy protein LC3-I and LC3-II were also increased (P<0.05). Conclusion: The expression level of lncRNA-BANCR affects the proliferation, invasion and apoptosis of thyroid cancer cells through modulation of autophagy behavior.