熊果酸拮抗巨噬细胞促进肝癌细胞迁移和侵袭的作用及其机制

目的：研究熊果酸对巨噬细胞-肝癌细胞共培养体中肝癌细胞迁移和侵袭能力的影响，并初步探讨其作用 机制。方法：采用Transwell迁移和侵袭试验检测熊果酸处理与非处理的巨噬细胞-肝癌细胞共培养体中肝癌细胞的迁 移和侵袭能力的变化；Western印迹法检测共培养体中肝癌细胞上皮-间质转化蛋白E-cadherin，N-cadherin和vimentin的 表达情况。结果：共培养后的肝癌细胞的迁移和侵袭能力显著增强，并发生了上皮-间质样表型转变，上皮-间质转化 的标志蛋白E-cadherin的表达下调，而N-cadherin和vimentin的表达均上调；熊果酸处理的巨噬细胞-肝癌细胞共培养体 中，肝癌细胞的迁移和侵袭能力降低，E-cadherin表达上调，N-cadherin和vimentin的表达下调。结论：熊果酸能够显 著抑制共培养体中肝癌细胞的迁移和侵袭能力及上皮-间质转化能力。

Effect of ursolic acid on invasion and migration of hepatocellular carcinoma cells co-cultured with macrophages and the underlying mechanisms

Objective: To investigate the eff ect of ursolic acid on the invasion and migration of hepatocellular carcinoma (HCC) cells co-cultured with macrophages, and to explore the underlying mechanisms. Methods: Th e migration and invasion ability of HCC cells in the co-culture system with or without ursolic acid intervention were evaluated by transwell assay. Th e levels of epithelial-mesenchymal transition (EMT) markers E-cadherin, N-cadherin, and vimentin in HCC cells co-cultured with macrophages were detected by Western blot. Results: The migration and invasion ability and EMT were significantly enhanced when co-cultured with macrophages, and the expression of E-cadherin was significantly increased while N-cadherin and vimentin levels were significantly decreased. However, after ursolic acid treatment, the migration and invasion ability were significantly reduced, and the expression of E-cadherin was increased while N-cadherin and vimentin levels were decreased. Conclusion: Ursolic acid exerts inhibitory effect on the ability of migration, invasion, and EMT for HCC, which are enhanced by co-culturing with macrophages.