BEZ235对体外前列腺癌细胞的抑制作用

目的：探讨PI3K/mTOR双重靶向抑制剂BEZ235对人前列腺癌细胞株的增殖、迁移能力的影响。方法：采用MTT实验分析BEZ235对正常前列腺上皮细胞RWPE-1和前列腺癌细胞PC3及DU145增殖能力的影响；划痕试验检测BEZ235对RWPE-1，PC3及DU145细胞迁移的影响；Western印迹及免疫荧光染色方法检测BEZ235对上皮-间质转化(epithelial-mesenchymal transition，EMT)相关标志物蛋白表达的影响。结果：BEZ235对PC3和DU145细胞的增殖具有明显的抑制作用(P<0.01)，而对RWPE-1作用不明显；BEZ235明显上调PC3和DU145细胞标志物E-cadherin的表达，下调间质标志物Vimentin的表达(P<0.01)。结论：BEZ235可体外抑制PC3和DU145细胞增殖和迁移，其部分机制可能是通过抑制EMT途径而实现。

Inhibitory effect of BEZ235 on human prostate carcinoma#br#in vitro

Objective: To determine effects of BEZ235, an inhibitor of phosphoionsitol-3-kinase (PI3K)/mTOR, on the cell proliferation and migration in human prostate carcinoma lines including RWPE-1, PC3, and DU145 cells.Methods: Viability of RWPE-1, PC3, and DU145 cells was detected by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT ) assay, while cell migration was analyzed by woundhealing assay. Western blot and immunofl uorescence were used to examine the changes of relevantprotein expression.Results: Th e proliferation of PC3 and DU145 cells was eff ectively inhibited by BEZ235 (P<0.01),whereas RWPE-1 was not obviously inhibited. Invasion and migration of PC3 and DU145 cellswere attenuated by BEZ235 via EMT pathway.Conclusion: The PI3K/mTOR dual inhibitor BEZ235 shows substantial anti-tumor activity inhuman prostate carcinoma lines of PC3 and DU145 cells, which may be involved in the EMTpathway.