Schiff碱稀土金属配合物对辐射导致肿瘤细胞DNA损伤及修复的影响

目的 Schiff碱具有抗瘤抑菌活性, 其抗癌作用与其对肿瘤细胞DNA的破坏有关。方法 利用脉冲电场凝胶电泳(PFGE)的方法, 观察了某种Schiff碱稀土金属化合物对肿瘤细胞DNA双链断裂及修复的影响。结果 该化合物能够增加辐射导致的DNA双链断裂的生成, 并抑制其修复。结论 Schiff碱稀土金属配合物能提高肿瘤细胞对辐射的敏感性, 为开发放化疗修饰剂提供了新的研究方向。

Effects of rare earth complexes with Schiff base on radiation induced DNA damage and repair of tumor cells

Objective To examine the effects of rare earth complexes with Schiff base on DNA double strand breaks and repair induced by radiation. Methods Using pulsed field gel electrophoresis (PFGE) to detect the DNA dsb induced by different dose irradiation,the integrated OD (Int OD%) of DNA migrated into the gel were measured by the software OneDScan of Biological Medical Electrophoresis Image Progress Analytic System (BMIPAS). Results LR1 treatment before irradiation could increase DNA dsb formation of SGC-7901 cells, and inhibit the process of DNA damage repair. Conclusion As a rare earth complex with Schiff base,LR1 can increase the radiosensitivity of SGC-7901 cells. For exploiting new radiochemotherapeutic modifiers,further research on these compounds is deserved.