| 重组人胰高血糖素样肽1(7-36)促进INS-1细胞胰岛素释放与合成 |
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| 引用本文： | 巩秋红,娄晋宁,叶丽亚,李光伟. 重组人胰高血糖素样肽1(7-36)促进INS-1细胞胰岛素释放与合成[J]. 中华内分泌代谢杂志, 2004, 20(6): 559-560 |
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| 作者姓名： | 巩秋红 娄晋宁 叶丽亚 李光伟 |
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| 作者单位： | 1. 100029,北京中日友好医院内分泌科
2. 中日友好临床研究所病理生理室 |
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| 基金项目： | 国家 8 63课题基金资助项目 (2 0 0 2AA2 0 50 61 ) |
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| 摘 要： | 在INS 1细胞中分别加入不同浓度的葡萄糖和重组人胰高血糖素样肽 1〔rhGLP 1(7 3 6〕溶液 ,孵育 4h ,用放免法测定上清液中胰岛素含量 ,并利用RT PCR技术对INS 1细胞的胰岛素mRNA水平作半定量分析。结果提示rhGLP 1(7 3 6)不仅能促进INS细胞的胰岛素释放 ,而且能促进胰岛素基因表达。
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| 关 键 词： | 胰高血糖素样肽1 INS-1细胞 胰岛素 糖尿病 |
| 修稿时间： | 2003-10-21 |
Recombinant human glucagon-like peptide-1 (7-36) enhances insulin release and insulin mRNA expression in INS-1 cells |
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| GONG Qiu-hong ,LOU Jin-ning,YE Li-ya,LI Guang-wei. Recombinant human glucagon-like peptide-1 (7-36) enhances insulin release and insulin mRNA expression in INS-1 cells[J]. Chinese Journal of Endocrinology and Metabolism, 2004, 20(6): 559-560 |
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| Authors: | GONG Qiu-hong LOU Jin-ning YE Li-ya LI Guang-wei |
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| Affiliation: | GONG Qiu-hong *,LOU Jin-ning,YE Li-ya,LI Guang-wei. *Department of Endocrinology,China-Japan Friendship Hospital,Beijing 100029 |
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| Abstract: | In INS-1 cells, the insulin secretion was investigated by radioimmunoassay (RIA) after 4 h incubation in medium containing different concentrations of glucose and recombinant human glucagon-like peptide-1 (rhGLP-1) (7-36). Insulin mRNA level in INS-1 cells was assessed by a semi-quantitative RT-PCR method. rhGLP-1 (7-36) is not only a powerful insulin secretagogue, but also can increase insulin gene expression in INS-1 cells. |
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| Keywords: | Glucagon-like peptide-1 INS-1 cell Insulin Diabetes mellitus |
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