| 伤寒杆菌耐药质粒pRST98毒力基因spv的序列测定 |
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| 引用本文： | 焦旸,黄瑞,祁小飞.伤寒杆菌耐药质粒pRST98毒力基因spv的序列测定[J].苏州大学学报(自然科学版),2004,24(2):130-133. |
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| 作者姓名： | 焦旸 黄瑞 祁小飞 |
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| 作者单位： | 苏州大学医学院微生物学教研室,江苏苏州215007 |
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| 摘 要： | 目的 对伤寒杆菌多重耐药质粒pRST98的毒力基因spv进行克隆及核酸序列测定。方法 为区分毒力基因存在于细菌染色体还是位于耐药质粒pRST98，先分离提取耐药质粒pRST98，再以该质粒为模板，将自行设计的引物经多聚酶链式反应(PCR)扩增目的基因，装入克隆载体pGEM-T EASY进行核酸序列测定。结果 PCR反应得到spv，基因的spvR和spvB片段，其ORF大小分别为894bp和1776bp，核酸序列测定后进行生物信息学分析。结论 伤寒杆菌多重耐药质粒pRST98除编码细菌抗药性外，还具有与细菌毒力相关的基因，该质粒是一种具有双重功能的“嵌合型”质粒。
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| 关 键 词： | 伤寒杆菌 耐药质粒 毒力基因 序列测定 |
| 文章编号： | 1000-5749(2004)02-0130-04 |
| 修稿时间： | 2003年10月30 |
DNA Sequencing of Virulence Gene spv on the Salmonella typhi Multidrug Resistance Plasmid pRST98 |
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| JIAO Yang,HUANG Rui,QI Xiao-fei.DNA Sequencing of Virulence Gene spv on the Salmonella typhi Multidrug Resistance Plasmid pRST98[J].Suzhou University Journal of Medical Science,2004,24(2):130-133. |
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| Authors: | JIAO Yang HUANG Rui QI Xiao-fei |
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| Abstract: | Objective To clone and sequence the virulence gene spv presented on Salmonella typhi multidrug resistance plasmid pR ST98 . Methods The spv gene was amplified by PCR(Polymerase Chains Reaction),and then ligated to plasmid vector pGEM-T EASY.The open reading frame(ORF) spvR and spvB were sequenced. Results The ORF of spvR sized 894bp,and spvB was 1776bp.The aquired DNA data was then analysed by bio_informatics. Conclusion Except for the ability to encode the drug resistance,the Salmonella typhi plasmid pR ST98 might also encode virulence.PCR and DNA sequencing results presented in this study support a mosaic-like structure for pR ST98 |
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| Keywords: | Salmonella typhi R plasmid virulence gene DNA sequence |
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