Instrumental and Bioanalytical Measures of Persistent Organochlorines in Blue Mussel (Mytilus edulis) from Korean Coastal Waters |
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Authors: | J S Khim D L Villeneuve K Kannan W Y Hu J P Giesy S-G Kang K-J Song C-H Koh |
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Institution: | (1) Department of Oceanography, Seoul National University, Seoul 151-742, Korea, KP;(2) National Food Safety and Toxicology Center, Department of Zoology, and Institute for Environmental Toxicology, Michigan State University, East Lansing, Michigan 48824-1311, USA, US |
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Abstract: | Blue mussels (Mytilus edulis) collected from 34 locations along the south and east coast of Korea were analyzed for polychlorinated biphenyls (PCBs) and
organochlorine (OC) pesticides. Maximum concentrations of PCBs and total OC pesticides were 98.5 and 20.5 ng/g, wet weight,
respectively. Extracts were fractionated by Florisil chromatography and each fraction was screened for dioxin-like activity
in vitro, using recombinant rat hepatoma cells (H4IIE-luc). Fraction 2 (F-2), which contained hexachlorocyclohexanes, chlordanes,
p,p′-DDD, and p,p′-DDT, generally elicited significant dioxin-like activity compared to control, whereas Fraction 1 (F-1), which contained
PCBs, p,p′-DDE, and hexachlorobenzene, did not. The greatest magnitude of dioxin-like response observed was 44% of the maximum response
elicited by a 2,000 pM 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) standard. The relatively low magnitudes of dioxin-like response observed for F-1 samples were consistent with
the relatively low PCB concentrations. At concentrations equal to the maximum observed in the mussel samples, neither individual
OC pesticides nor a mixture of OC pesticides yielded a significant dioxin-like response in the H4IIE-luc assay. Thus, the
concentrations of OC pesticides in F-2 did not appear to have accounted for the dioxin-like activity observed. This suggests
the presence of unidentified and/or unknown, acid-stable, dioxin-like compounds in F-2. This study suggests that in vitro bioassays are useful in assessing the contamination of mussels collected from coastal marine locations.
Received: 28 November 1999/Accepted: 3 May 2000 |
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