| SSV—NIH3T3细胞的无血清培养 |
| |
| 引用本文： | 高亚胜 田竟生. SSV—NIH3T3细胞的无血清培养[J]. 解剖学报, 1991, 22(2): 190-194,I015 |
| |
| 作者姓名： | 高亚胜 田竟生 |
| |
| 作者单位： | 北京市肿瘤防治研究所，北京市肿瘤防治研究所，河南医科大学组织学胚胎学教研室 河南洛阳华美生物工程公司 洛阳 471001 |
| |
| 摘 要： |
|
| 关 键 词： | 无血清培养 血小板 生长因子 细胞 |
SERUM-FREE CULTURE OF SSV-NIH3T3 CELLS |
| |
| Abstract: | The establishment of a serum-free culture medium (SFM) for SSV-NIH3T3 cells is reported in this paper. In this medium DMEM/Ham's F12 is the fundamental element in basal medium, insulin and undefatty bovine serum albumin are key elements among the supplements. In this SFM SSV-NIH3T3 cells grow well, they keep the ability of secreting platelet-derived growth factor-like material into culture medium and causing tumor growth in nude mice. |
| |
| Keywords: | Serum-free culture SSV-NIH_3T_3 cells Platelet-derived growth factor |
| 本文献已被 CNKI 维普 等数据库收录！ |
