| RNA干扰组蛋白去乙酰化酶1对人胰腺癌细胞增殖、凋亡的调控机制研究 |
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| 引用本文： | 高道键,徐岷,张玉琦,李雅洁,满晓华,吴红玉,金晶. RNA干扰组蛋白去乙酰化酶1对人胰腺癌细胞增殖、凋亡的调控机制研究[J]. 解放军医学杂志, 2010, 35(4) |
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| 作者姓名： | 高道键 徐岷 张玉琦 李雅洁 满晓华 吴红玉 金晶 |
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| 作者单位： | 1. 温州医学院附属第一医院消化内科,浙江温州,325000
2. 上海第二军医大学长海医院消化内科,上海,200433 |
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| 基金项目： | 温州市科技局资助项目(Y20090102) |
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| 摘 要： | 目的观察沉默组蛋白去乙酰化酶1(HDAC1)基因对人胰腺癌PaTu8988细胞增殖、凋亡的影响及可能机制。方法胰腺癌PaTu8988细胞株分为空白对照组(不予任何处理)、阴性对照siRNA组(予以30nmol/L阴性siRNA)、siRNA1组(予以15nmol/LHDAC1siRNA)和siRNA2组(予以30nmol/LHDAC1siRNA)。siRNA转染48h后,用相对实时定量RT-PCR法和West-ernblotting检测HDAC1基因表达情况;相对实时定量RT-PCR法检测细胞增殖凋亡相关基因p21、Bcl-2、Bax的表达;WST-8法检测细胞增殖情况;流式细胞术检测细胞凋亡变化。结果转染HDAC1siRNA48h后人胰腺癌PaTu8988细胞中HDAC1mRNA表达量在siRNA1组和siRNA2组分别为46.1%±6.1%和32.3%±1.4%,均显著低于空白对照组(100.0%±3.4%)和阴性对照siRNA组(87.4%±28.3%,P<0.05)。Westernblotting显示HDAC1蛋白表达量亦明显下降,以siRNA2组表达量最低(P<0.05)。WST-8法检测显示4组细...
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| 关 键 词： | 基因 HDAC1 胰腺肿瘤 RNA干扰 细胞增殖 细胞凋亡 |
Mechanism of RNA interference on proliferation and apoptosis regulation of pancreatic cancer cell line PaTu8988 by HDAC1 siRNA |
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| GAO Dao-jian,XU Min,ZHANG Yu-qi,LI Ya-jie,MAN Xiao-hua,WU Hong-yu,JIN Jing. Mechanism of RNA interference on proliferation and apoptosis regulation of pancreatic cancer cell line PaTu8988 by HDAC1 siRNA[J]. Medical Journal of Chinese People's Liberation Army, 2010, 35(4) |
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| Authors: | GAO Dao-jian XU Min ZHANG Yu-qi LI Ya-jie MAN Xiao-hua WU Hong-yu JIN Jing |
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| Abstract: | Objective To investigate the effects of histone deacetylase 1 (HDAC1) gene silencing on cell proliferation and apoptosis of pancreatic cancer cell line PaTu8988.Methods PaTu8988 cells were cultured and divided into four groups:control group (untreated),negative siRNA group (treated with 30nmol/L negative control siRNA),siRNA1 group (treated with 15nmol/L HDACl siRNA) and siRNA2 group (treated with 30nmol/L HDACl siRNA).RT-PCR and Western blotting were used to detect the gene expression of HDAC1 48 hours aft... |
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| Keywords: | gene HDAC1 pancreatic neoplasms RNA interference cell proliferation cell apoptosis |
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