全蝎注射液对大鼠原位性肾炎病理变化及血清IL-1的影响

本实验在大鼠C-BSA原位肾炎模型基础上,用全蝎注射液(1.0g/kg)每日1次腹腔注射,3周后杀检。取肾皮质作病理检查,用胸腺细胞增殖法检测血清白细胞介素1(IL-1)。结果发现:全蝎治疗组的尿蛋白含量比模型组明显减少(P<0.05),血清IL-1活性比模型组显著下降(P<0.01),光镜及电镜下病理变化也有所缓解。结果提示:全蝎注射液可能对大鼠C-BSA原位肾炎血清IL-1有抑制作用,并能缓解其病理改变过程。

Effects of Scorpion Injection on Interleukin 1 and Pathology in Experimental Glomerulonephritis in Situ

The model of experimental glomerulonephritis in situ in rats was established by administration of C-BSA. 24 male Wistar rats were randomly assigned to three groups: C-BSA, control and C- BSA scorpion (scorpion injection, ip, 1.0g/kg, once/day). At the end of 4th week, the bioactivity of serum IL-1 was assayed by thymocyte proliferation method and the renal pathology was checked up. Urine sample was collected for 24 hour once/week for protein determination. Result: Quantity of proteinuria in scorpion group was reduced significantly in comparing with C-BSA group. Level of IL-1 was significantly lower in scorpion group than that in C-BSA group. Injury of renal tissue also was mild in the treated group. These results suggested that scorpion injection could suppress the activities of IL-1 and ameliorate the pathological injury in this experimental glomerulonephritis in situ.