慢性肝脏疾病中CD3^-CD161^＋NK和CD3^＋CD161^＋NKT细胞亚群的变化研究

目的探讨CD3^-CD161^＋NK、CD3^＋CD161^＋NKT细胞在慢性肝炎／肝硬化及肝细胞癌患者肝脏组织及外周血中表达及意义。方法利用流式细胞仪对31例肝细胞癌患者、59例慢性肝炎／肝硬化患者肝脏组织及外周血、15例正常肝脏组织、48例正常人外周血中的CD3^-CD161^＋NK和CD3^＋CD161^＋NKT进行定量分析。结果慢性肝炎／肝硬化组CD3^-CD161^＋NK细胞[（13．4±1．3）％]和肝细胞癌癌周组CD3^-CD161^＋NK细胞[（16．7±4．8）％]及远离肝癌组的肝脏组织CD3^-CD161^＋NK细胞[（22．0±4．4）％]与正常肝脏组织CD3^＋CD161^＋NK细胞[（35．1±7．2）％]相比，肝细胞癌癌周肝脏组织内含量最低（t值分别为2．301、2．137、2．034，P〈0．05）；外周血中肝细胞癌组CD3^-CD161^＋NK细胞f（11．6±6．3％）]、CD3^＋CD161^＋NKT细胞[（14．7±6．2）％]与慢性肝炎／肝硬化组CD3^-CD161^＋NK细胞[（10．8±1．7）％]、CD3^＋CD161^＋NKT细胞[（12．5±0．8）％]、正常对照组CD3^＋CD161^＋NK细胞[（7．5±0．8）％]、CD3^-CD161^＋NKT细胞[（13．8±1．7）％]相比肝癌组CD3^-CD161^＋NKT细胞含量最高（t值分别为2．134，2．099，P〈0．05），肝癌组CD3^＋CD161^＋NKT细胞含量最高（t值分别为2．125，2．154，P〈0．05）。结论由于NK细胞及NKT细胞数量减少或／和活性降低，使肿瘤细胞逃逸了免疫监视，可能促进了肿瘤的发生、发展及转移。

Alterations of CD3~- CD161~+NK cell and CD3~+CD161~+NKT Cell population in the chronic hepatic diseases

Objective To investigate the significance of the alteration of NK cell population in hepatocellular carcinoma(HCC).Methods The number and distribution of CD3~-CD161~+NK cells,CD3~+CD161~+NKT cells in liver tissue(31 patients vs 15 healthy controls)and peripheral blood(59 patients vs 48 healthy controls)of chronic hepatitis/hepatic cirrhosis were analyzed by flow cytometry.Results CD3~-CD161~+NK cells[(16.7±4.8)%],in the surrounding tissue of HCC were significantly lower than far distancing hepatocellular carcinoma group[(22.0±4.4)%]、chronic hepatitis/hepatic cirhosis group[(13.4±1.3)%],and normal control group[(35.1±7.2)%](t=2.301,2.137,2.034,P<0.05).CD3~-CD161~+NK cells[(11.6±6.3)%]and CD3~+CD161~+NKT cells[(14.7±6.2)%]in peripheral blood of HCC were significantly higher than chronic hepatitis/hepatic cirrhosis[(10.8±1.7)%、(12.54±0.8)%]and normal control group [(7.5±0.8)%,(13.8±1.7)%],(t=2.134,2.099,P<0.05).Conclusion The number of NK cells and NKT cells in the surrounding tissue of HCC was decreased.This result may imply that tumor may escape from immunological surveillance mechanisms,leading to its advanced stages.