| 膀胱二聚体Cajal间质细胞的超微结构及其对ATP的电反应 |
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| 引用本文： | 王江平,王勤章,丁国富,倪钊,李应龙,王新敏,李强,钱彪,王文晓.膀胱二聚体Cajal间质细胞的超微结构及其对ATP的电反应[J].神经解剖学杂志,2012,28(6):617-620. |
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| 作者姓名： | 王江平 王勤章 丁国富 倪钊 李应龙 王新敏 李强 钱彪 王文晓 |
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| 作者单位： | 1. 石河子大学医学院第一附属医院泌尿外科,石河子832000;华中科技大学同济医学院附属同济医院泌尿外科,武汉430000
2. 石河子大学医学院第一附属医院泌尿外科,石河子,832000 |
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| 基金项目： | 国家自然科学基会资助项目(81060064) |
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| 摘 要： | 目的:为证明膀胱二聚体Cajal间质细胞(interstitial cells of Cajal,ICC)发挥起搏作用提供超微结构和功能学证据。方法:电子显微镜下鉴定组织和培养的豚鼠膀胱二聚体ICC并观察ICC之间的超微联系;体外分离、培养豚鼠膀胱ICC,运用细胞膜片钳技术观察ATP(100μmol/L)刺激下二聚体ICC和单体ICC的兴奋性差别。结果:二聚体ICC中两个ICC细胞胞体紧密相连,细胞之间的间隙小于20 nm,存在大量缝隙连接,局部细胞膜融合,形成联胞体。在ATP(100μmol/L)的刺激下体外培养的二聚体ICC的内向电流幅度为286.9±26.4 pA,显著大于单体ICC的内向电流幅度163.8±18.6 pA(P<0.01)。结论:二聚体ICC具有形成高兴奋起搏电流的超微结构基础,高兴奋性的二聚体ICC可能才是逼尿肌的起搏细胞。
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| 关 键 词： | Cajal间质细胞 起搏细胞 膀胱 豚鼠 |
Ultrastructure and responses to ATP of dimmer interstitial cells of Cajal in bladder |
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| Wang Jiangping,Wang Qinzhang,Ding Guofu,Ni Zhao,Li Yinglong,Wang Xinmin,Li Qiang,Qian Biao,Wang Wenxiao.Ultrastructure and responses to ATP of dimmer interstitial cells of Cajal in bladder[J].Chinese Journal of Neuroanatomy,2012,28(6):617-620. |
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| Authors: | Wang Jiangping Wang Qinzhang Ding Guofu Ni Zhao Li Yinglong Wang Xinmin Li Qiang Qian Biao Wang Wenxiao |
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| Institution: | 1(1.Department of Urology,the First Affiliated Hospital of Medical College,Shihezi University,Shihezi 832000; 2.Department of Urology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430000,China) |
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| Abstract: | Objective: To confirm dimmer interstitial cells of Cajal(ICC) in bladder are pacemaker cells with the ultrastructural and functional evidences.Methods: Dimmer ICC of guinea pig bladder were identified and the contacts between two single cells of dimmer ICC were observed by electron microscopy.The difference of excitability,elicited by ATP(100 μmol/L),between dimmer ICC and monomer ICC isolated and cultured in vitro,was investigated by using patch clamp technique.Results: Two single cell bodies of dimmer ICC were closely in contact,the gap between the cells is less than 20 nm.There were a large number of gap junctions between two single cells of dimmer ICC.The boundaries were blurred between the local cell membrane.The inward current of dimmer ICC(286.9±26.4 pA),stimulated by ATP(100 μmol/L),was significantly greater than that of monomer ICC(163.8±18.6 pA,P<0.01).Conclusion: Dimmer ICC in bladder have the ultrastructural basis of pacemaker current and are likely to be pacemaker cells to excite detrusor. |
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| Keywords: | interstitial cells of Cajal pacemaker cell bladder guinea pig |
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