益骨胶囊含药血清在共育体系中对大鼠成骨细胞增殖、ALP 活性及IL-11 mRNA表达的影响

目的：观察益骨胶囊含药血清在成骨-破骨细胞共育体系中对SD大鼠成骨细胞(OB)增殖、碱性磷酸酶（ALP）活性及白细胞介素-11(IL- 11)mRNA表达的影响。方法：(1)取1d龄SD大鼠颅骨分离培养OB，取5 d龄 SD大鼠四肢股骨、胫骨分离培养破骨细胞(OC)，建立细胞上清相通但细胞间不相互混杂的平 面式成骨- 破骨细胞共育体系，实验分为两组(含药血清组和对照组)。 (2) MTT法检测OB增殖，氨基安替吡啉测酚法测定ALP活性，FQ-PCR法测定IL-11 mRNA相对表达量。结果：含药血清组中OB的A值、ALP活性、IL-11/β-actin比值均高于对照组(P<0.05) 。结论：益骨胶囊含药血清在共育体系中可促进OB增殖、增强OB ALP活 性，提高IL-11 mRNA的表达。

Effect of yigu capsule drug-containing serum on proliferation,ALP activity and expression of interleukin-11 mRNA in rat osteoblasts in the co- culture

AIM: To investigate the effect of yigu capsule drug-containing serum on proliferation, ALP activity and expression of interleukin-11 mRNA in Sprague-Dawley rat osteobalsts in the co-culture system. METHODS: (1) Osteoblasts and osteoclasts were isolated from 1 and 5-day-old Sprague-Dawley rats, respectively. The osteoblasts-osteoclasts co-culture system was built to prevent the two kinds of cells from contact and allow the media to exchange. The experiment included two groups, drug-containing sera group and control group. (2) The osteoblasts proliferation, ALP activity and expression of interleukin-11 mRNA were detected by the MTT, 4-aminoantipyrine spectrometric methods and FQ-PCR,respectively. RESULTS: In drug-containing sera group, the osteoblasts proliferation, ALP activity, expression of IL-11 mRNA were higher than those of control group (P<0.05). CONCLUSION: Rat sera containing yigu capsule can obviously enhance the osteoblast proliferation, ALP activity and expression of IL-11 mRNA.