Survivin蛋白表达与胃癌发生发展的关系及意义--组织芯片技术研究

目的探讨凋亡抑制基因Survivin编码蛋白表达与胃癌发生发展的关系及其相关分子病理学机制。方法采用组织芯片仪(M icroarrayer,Beecher Instrum ents,USA)构建含98例胃癌及其癌前病变的直径1.0 mm的225个微组织阵列芯片,免疫组织化学方法检测分析survivin蛋白表达情况。结果胃癌及其癌前病变组织芯片蜡块组织阵列排列整齐,常规切片组织形态良好。凋亡抑制基因survivin编码蛋白在胃癌组织中的表达阳性率显著高于正常胃黏膜、肠上皮化生、不典型增生组织中的表达(P<0.01),胃癌转移组survivin蛋白表达阳性率显著高于无转移组(P<0.05)。Survivin蛋白表达与胃癌病理分期、组织学类型和大体类型没有相关性(P>0.05)。结论组织芯片技术在人胃癌早期诊断中具有其他组织病理学技术不可替代的高效、省时、低消耗的优点。Survivin在胃黏膜癌变过程中被激活而参与胃癌的发生和发展,可作为一个较为理想的肿瘤标志物用于胃癌的早期诊断和转移的预警。

The Relationship of Survivin Protein Expression with Carcinogenesis and Progression of Gastric Carcinoma-A Study by Tissue-microarray

Objective To explore the relationship of survivin protein expression with the molecular mechanism of carcinogenesis and progression of gastric carcinoma.Methods Two blocks of tissue microarray were constructed,one containing 124 and another containing 101 small cylindrical samples,1.0 mm each in diameter,from 96 cases of gastric carcinoma and the precancerous lesions.The immunohistochemical stainning method was used to detect the survivin protein expression.Results The positive rate of survivin gene encoding protein in gastric cancer tissues was significantly higher than those in adjacent normal mucosa,intestinal metaplasia and dysplasia(P<0.01).The positive rate of survivin protein expression in tumors with metastases was statistically higher than that in tumors without metastasis(P<0.05).The expression of survivin was not related to the clincopathological stage,histological classification,lauren′s type and gross type of gastric cancer(P>0.05).Conclusions Tissue microarray is a new powerful technique with a conspicuous characteristic of small volume rich in information in the tumor investigation.Survivin gene locked-up in normal human tissues is activated and involved in tumorigenesis and progression of gastric cancers and survivin can be a ideal marker for early diagnosis of gastric carcinoma and the metastasis.